Replication protein

Bochkarev, A., Pfuetzner, R. A., Edwards, A. M., and Frappier, L. (1997). Structure of the single-stranded-DNA-binding domain of replication protein A bound to DNA. Nature 85, 176-181. 

Gonciarz-Swiatek, M. et al. Recognition, targeting, and hydrolysis of the lambda O replication protein by the ClpP/ClpX protease. / Biol Chem 1999, 274, 13999-4005. 

Hovanessian AG, Puvion-Dutilleul F, Nisole S, Svab J, Perret E, Deng JS, Krust B (2000) The cell-surface-expressed nucleolin is associated with the actin cytoskeleton. Exp Cell Res 261 312—328 Iftode C, Daniely Y, Borowiec JA (1999) Replication protein A (RPA) the eukaryotic SSB. Crit Rev Biochem Mol Biol 34 141-180... 

Costello, E., Saudan, P., Winocour, E., Pizer, L., and Beard, P. (1997) High mobility group chromosomal protein 1 binds to the adeno-associated virus replication protein (Rep) and promotes... 

Molecular complexation is a precondition for receptor functions such as substrate selection, substrate transportation, isomeric differentiation, and stereoselective catalysis. Although the investigation of such functions with synthetically derived compounds is a relatively new development in chemistry, they are well known and extensively studied functions in the biological domain. Evolution, gene expression, cell division, DNA replication, protein synthesis, immunological response, hormonal control, ion transportation, and enzymic catalysis are only some of the many examples where molecular complexation is a prerequisite for observing a biological process. 

Two other protein complexes also function in eukaryotic DNA replication. RPA (replication protein A) is a eukaryotic single-stranded DNA-binding protein, equivalent in function to the E. coli SSB protein. RFC (replication/actor (J) is a clamp loader for PCNA and facilitates the assembly of active replication complexes. The subunits of the RFC complex have significant sequence similarity to the subunits of the bacterial clamploading (y) complex. 

Cells of patients with Bloom syndrome (BS) have many chromosome breaks and a high frequency of sister chromatid exchanges, perhaps in an effort to correct these breaks. The body is small but well-proportioned.kk A somewhat similar disease, the Werner syndrome (WS), is associated with premature aging.11 The Bloom s protein BLM and the WS gene product WRN are both helicases related to E.coli RecQ. Protein BLM colocalizes with replication protein A as discrete foci in the meiotic synaptonemal complex.1 3 Protein WRN also seems to be associated with DNA replication. Defects... 

Plasmid incompatibility is a situation where plasmids that replicate themselves in similar ways cannot coexist in the same bacterial cell because of forced competition for replication proteins and RNA. Apramycin 18 mimics the function of a small piece of RNA that dictates incompatibility with an ampicillin-resistance plasmid and triggers the eviction of those plasmids from the bacterial cells rendering them susceptible to ampicillin treatment. 

Caridha D, Kathcart AK, Jirage D, Waters NC (2010) Activity of substituted thiophene sulfonamides against malarial and mammalian cyclin dependent protein kinases. Bioorg Med Chem Lett 20(13) 3863-3867 Geyer JA, Keenan SM, Woodard CL et al (2009) Selective inhibition of Pfinrk, a Plasmodium falciparum CDK, by antimalarial l,3-diaryl-2-propenones. Bioorg Med Chem Lett 19(7) 1982-1985 Jirage D, Chen Y, Caridha D et al (2010) The malarial CDK Pfinrk and its effector PfMATl phosphorylate DNA replication proteins and co-localize in the nucleus. Mol Biochem Parasitol 172(1) 9-18... 

Current theories propose that the development of cell membranes accelerated early life. Enclosing chemicals responsible for replication, protein synthesis and energy generation within a confined space allows these essential life processes to occur far more frequently than they would do otherwise. Biological membranes comprise two phospholipid layers held together by interdigitation of their hydrophobic alkyl tails as shown in Fig. 5.1. They form a lamellar structure with their polar internal and external surfaces separated by a non-polar region. In their extended form each... 

Zylicz, M., Ang, D., Liberek, K., Yamamoto, T., and Georgopoulos, C. (1988). Initiation of lambda DNA replication reconstituted with purified lambda and Escherichia coli replication proteins. Biochim. Biophys. Acta 951, 344-350. 

Human replication protein A Replication ILIO CysL 2 Cysp 13 Cysp 4 Cysp (N)... 

Capsid (Xie etal, 2002), nuclease domain of Rep (replication protein) (Hickman etal, 2002)... 

Gao D, McHenry CS. tan binds and organizes Escherichia coli replication proteins through distinct domains. Domain IV, located 61. within the unique C terminus of tan, binds the replication fork, helicase, DnaB. J. Biol. Chem. 2001 276 4441-4446. 

Walter J, Newport J. Initiation of eukaryotic DNA replication origin unwinding and sequential chromatin association of Cdc45, RPA, and DNA polymerase alpha. Mol. Cell 2000 5 617-627. Zou L, Stillman B. Assembly of a complex containing Cdc45p, replication protein A, and Mcm2p at replication origins controlled... 

Zou L, Liu D, Elledge SJ. Replication protein A-mediated recruitment and activation of Radl7 complexes. Proc. Natl. Acad. Sci. 168. U.S.A. 2003 100 13827-13832. 

---