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Origins of replication

At the origin of replication (ori), there is an association of sequence-specific dsDNA-binding proteins with... [Pg.326]

ARS Autonomously replicating sequence the origin of replication in yeast. [Pg.413]

A hypercycle is a more complex organisation form. Its precondition is the presence of several RNA quasi-species which are able to amalgamate chemically with certain proteins (enzymes or their precursors). If such a protein is linked to a quasi-species, the resulting duo favours the replication of a second quasispecies. According to Dyson, the linked populations get stuck in a stable equilibrium. Problems occur at this level Any theory on the origin of replication has the central problem that the replication process must occur perfectly in order to ensure survival . If there are replication errors, these will increase from generation to generation, until the system collapses the error catastrophe has then occurred ... [Pg.223]

Technically, cDNAs are cloned into plasmids carrying an antibiotic selection marker for propagation in bacteria, a nutritional selection marker for selection of transfected yeast as well as the respective origins of replication. After amplification in bacteria, the plasmids are transfected into the corresponding auxothrophic yeast strain using similar methods as for bacteria and stably... [Pg.591]

The bacterial chromosome is a closed, double-stranded circular DNA molecule having a single origin of replication. Separation of the two parental strands of DNA creates two replication forks that move away from each other in opposite directions around the circle. Replicatioii is,... [Pg.15]

Each eukaryotic chromosome contains one linear molecule of DNA having multiple origins of replication. Bidirectional replication occurs by means of a pair of replication forks produced at each origin. Completion of the process results in the production of two identical linear molecules of DNA. DNA replication occurs in the nucleus during the S phase of the eukaryotic cell cycle. The two identical sister chromatids are separated om each other when the ceU divides during mitosis. [Pg.16]

The base sequence at the origin of replication is recognized and bound by the dna A protein. The two parental strands of DNA are pulled apart to form a replication bubble. ... [Pg.17]

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Origin of replication in eukaryotes

The origins of replication

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