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Replication Escherichia coli

Zylicz, M., Ang, D., Liberek, K., Yamamoto, T., and Georgopoulos, C. (1988). Initiation of lambda DNA replication reconstituted with purified lambda and Escherichia coli replication proteins. Biochim. Biophys. Acta 951, 344-350. [Pg.98]

Gao D, McHenry CS. tan binds and organizes Escherichia coli replication proteins through distinct domains. Domain IV, located 61. within the unique C terminus of tan, binds the replication fork, helicase, DnaB. J. Biol. Chem. 2001 276 4441-4446. [Pg.82]

The chromosomes of Escherichia coli and other bacteria are single, double-stranded DNA molecules with a total length of more than 1,000 pm. Relaxed DNA exists as a helical molecule, with one full turn of the helix occurring approximately every 10.4 base pairs. This molecule must undergo several folding and compaction steps to fit into an E. coli cell which is only 1-3 pm long. Despite this enormous compaction, bacterial DNA must be accessible for the bacterial enzymes that catalize DNA replication and transcription... [Pg.1056]

Induced mutagenesis in Escherichia coli is an active process involving proteins with DNA replication, repair, and recombination functions. The available evidence suggests that mutations are generated at sites where DNA has been damaged and that they arise via an error-prone repair activity. In an attempt to understand what specific contributions to mutagenesis are made by DNA lesions, we have studied the mutational specificity of some carcinogens, such as benzo[a]pyrene and aflatoxin, whose chemical reactions with DNA are... [Pg.330]

Investigations of pure cultures of bacteria clearly show the existence of a threshold concentration for the carbon source below which replication does not occur. This value is about 18 pg/1 for Escherichia coli and Pseudomonas sp. growing on glucose, 180 pg/1 for Aeromonas hydrophila growing on starch,... [Pg.355]

Although replication in prokaryotes is now well understood, many details in eukaryotes are still unclear. However, it is certain that the process is in principle similar. A simplified scheme of replication in the bacterium Escherichia coli is shown here. [Pg.240]

Chin YE, Snow ET, Christie NT. 1994. The stimulatory effect of nickel chloride on DNA replication in human HeLa cells and Escherichia coli. Carcinogenesis 15(5) 1013-1016. [Pg.227]

Burland, V. Plunkett, G. Daniels, D.L. Blattner, F.R. DNA sequence and analysis of 136 kilobases of the Escherichia coli genome organizational symmetry around the origin of replication. Genomics, 16, 551-561 (1993)... [Pg.460]

Hill, T.M. Tecklenburg, M.L. Pelletier, A.J. Kuempel, P.L. Tus, the transacting gene required for termination of DNA replication in Escherichia coli, encodes a DNA-binding protein. Proc. Natl. Acad. Sci. USA, 86, 1593-1597 (1989)... [Pg.463]

Katayama, T. (2001) Feedback controls restrain the initiation of Escherichia coli chromosomal replication. Mol. Microbiol. 41, 9-17. [Pg.992]

Marians, K.J. (2000) PriA-directed replication fork restart in Escherichia coli. Trends Biochem. Sci. 25, 185-189. [Pg.993]

Replication of the Escherichia coli Chromosome Initiation and Termination of Escherichia coli Chromosomal Replication DNA Replication in Eukaryotic Cells Eukaryotic Chromosomal DNA SV40 Is Similar to Its Host in Its Mode of Replication... [Pg.650]

Source Adapted from T. A. Baker and S. H. Wickner, Genetics and enzymology of DNA replication in Escherichia coli. Ann. Rev. Genetics, 26 447, 1992. [Pg.655]

Initiation and Termination of Escherichia coli Chromosomal Replication... [Pg.660]

As an example, we use RNAI, an antisense repressor of the replication of some Escherichia coli plasmids with a ColEl origin of replication. [Pg.184]

The first successful attempts to study RNA evolution in vitro were carried out in the late sixties by Sol Spiegelman9 and his group at Columbia University (Spiegel-man, 1971). They made use of an RNA replicase isolated from Escherichia coli cells infected by the RNA bacteriophage QP and prepared a medium for replication by adding the four ribonucleoside triphosphates (GTP, ATP, CTP, and UTP) in a suitable buffer solution. QP RNA, when transferred into this medium, instantaneously started to replicate. Evolutionary experiments were carried out by means of the serial transfer technique (Figure 4). Materials consumed in RNA replication... [Pg.171]

Figure 4. The technique of serial transfer. An RNA sample which is capable of replication in the assay is transferred into a test-tube containing stock solution. This medium contains the four nucleoside triphosphates (ATP, UTP, GTP and CTPJand a virus specific RNA polymerase, commonly QP-replicase because of the stability of this protein, in a suitable buffer solution. RNA replication starts instantaneously. After a given period of time a small sample is transferred to the next test-tube and this procedure is repeated about one hundred times. The transfer has two consequences (i) the material consumed in the replication is replaced, and (ii) the distribution of RNA variants is subjected to a constraint selecting for the fastest replicating species. Indeed, the rate of replication is increased by several orders of magnitude in serial transfer experiments starting out from natural QB RNA and leading to variants that are exclusively suited for fast replication and hence are unable to infect their natural hosts, Escherichia coli. Figure 4. The technique of serial transfer. An RNA sample which is capable of replication in the assay is transferred into a test-tube containing stock solution. This medium contains the four nucleoside triphosphates (ATP, UTP, GTP and CTPJand a virus specific RNA polymerase, commonly QP-replicase because of the stability of this protein, in a suitable buffer solution. RNA replication starts instantaneously. After a given period of time a small sample is transferred to the next test-tube and this procedure is repeated about one hundred times. The transfer has two consequences (i) the material consumed in the replication is replaced, and (ii) the distribution of RNA variants is subjected to a constraint selecting for the fastest replicating species. Indeed, the rate of replication is increased by several orders of magnitude in serial transfer experiments starting out from natural QB RNA and leading to variants that are exclusively suited for fast replication and hence are unable to infect their natural hosts, Escherichia coli.
Kroeger KM, Kim J, Goodman MF, Greenberg MM (2004b) Effect of the C4 -oxidized abasic site on replication in Escherichia coli. An unusually large deletion is induced by a small lesion. Biochemistry 43 13621-13627... [Pg.463]

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See also in sourсe #XX -- [ Pg.240 , Pg.241 ]

See also in sourсe #XX -- [ Pg.798 , Pg.799 , Pg.800 ]



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