Recombinant nuclear polyhedrosis virus

Table L Recombinant Nuclear Polyhedrosis Viruses Constructed for Increased Insecticidal Activity...

Development and Characterization of a Recombinant Nuclear Polyhedrosis Virus, AcAalT... 

Kondo, A. and Maeda, S. (1991) Host range expansion by recombination of the baculoviruses Bombyx mori nuclear polyhedrosis virus and Autographa californica nuclear polyhedrosis virus. Journal of Virology, 65 (7), 3625-3632. 

Baculovirus expression is the most frequently used method for expression in insect cells and employs Autographa californica nuclear polyhedrosis virus (AcNPV), a double stranded (ds) DNA virus that infects arthropods. The baculovirus expression system utilizes features of the viral life cycle to introduce recombinant DNA coding the gene of interest into insect cells (Miller, 1988 O Reilly et al, 1992). 

The baculoviruses which have received the majority of attention to date are two of the nuclear polyhedrosis viruses (NPV). One was originally derived from the alfalfa loopcr Autographa californica, and the other from the silkworm Bombyx mori. These viruses are referred to as AcNPV and BmNPV respectively. The engineering of baculoviruses to express JHE was undertaken in both of these systems by a number of different groups. Details of methodology for construction and purification of recombinant baculoviruses are given elsewhere (28,31). 

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