Gene replacement

Manstein, D.J., Titus, M.A., DeLozanne, A., and Spudich, J.A. (1989). Gene replacement in Dic-tyostelium Generation of myosin null mutants. EMBO J. 8, 923-932. 

The somatic cell gene replacement described above would obviously not be passed on to offspring. Other strategies to alter germ cell lines have been devised but have been tested only in experimental animals. A certain... 

Although much progress has been made in gene replacement therapy, significant challenges remain. These include ... 

For loss-of-fundion mutations gene replacement therapy usng retroviruses, adenoviruses, adeno-associated viruses, or liposomes as vectors... 

Gene replacement therapy for loss of function mutations... 

Roth JA, Grammer SF, Swisher SG, et al. Gene replacement strategies for treating non-small cell lung cancer. Semin Radiat Oncol 2000 10(4) 333-342. 

Roth J A, Swisher SG, Merritt J A, etal. Gene therapy for non-small cell lung cancer a preliminary report of a phase I trial of adenoviral p53 gene replacement. Semin Oncol 1998 25 33-37. 

Roth, J.A., S.F. Grammer, S.G. Swisher, R. Komaki, J. Nemunaitis, J. Merritt, and R.E. Meyn, P53 gene replacement for cancer— interactions with DNA damaging agents. Acta Oncol, 2001.40(6) 739-44. 

Land KM, Delgadillo-CorreaMG, Tachezy J, Vanacova S, Hsieh CL, Sut ak R, Johnson PJ (2004) Targeted gene replacement of a ferredoxin gene in Trichomonas vaginalis does not lead to metronidazole resistance. Mol Microbiol 51 115-122... 

Knockout mice. If targeted DNA is injected into a fertilized mouse egg, there is a chance that the mouse will have the targeted gene replaced in one chromo-... 

Various protocols have been used to treat cancer by gene therapy using adenoviruses, which include gene-based immunotherapy, prodrug therapy and gene replacement approaches. These procedures are often employed in combination with... 

Chen, C., Nagy, Z., Luning Prak, E., Weigert, M. (1995). Immunoglobulin heavy chain gene replacement a mechanism of receptor editing. Immunity 3,747-755. 

Usuda, S., Takemori, T., Matsuoka, M., Shirasawa, T., Yoshida, K., Mori, A., Ishizaka, K., Sakano, H. (1992). Immunoglobulin V gene replacement is caused by the intramolecular DNA deletion mechanism. EMBO J. 11,611-618. 

Figure 4 Dehydratase (DH) consensus sequence of avermectin module 10 (inactive), module 9 (active), module 2, and erythromycin DH domains. Amino acids listed in the consensus line in capital letters are shared by all 4 DHs, and those in lower case are shared by 3 of the 4 regions. The underlined module 10 region was inserted into module 2 or the underlined module 9 region was inserted into module 2 for gene replacement experiments. The ( ) sequences are the conserved HxxxGxxxxP active domain.

C Khosla, S Ebert-Khosla, DA Hopwood. Targeted gene replacements in a Streptomyces polyketide synthase gene cluster role for the acyl carrier protein. Mol Microbiol 6 3237-3249, 1992. 

Batten s CLN3P Gene replacement Global Hackett et al. (2004) Rakheja et al. (2004)... 

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