Rearrangements Recombinant bacteria

However, the YAC-based technique is more labor intensive. The yield of pure YAC DNA is only a small fraction of the amount of the PI DNA that can be obtained from bacteria, and the protocol uses a two-step pop-in, pop-out strategy. Furthermore, the efficient machinery for homologous recombination in the yeast can, unfortunately, give rise to spontaneous rearrangement of the DNA. 

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