Poly-3-hydroxybutyrate using recombinant bacteria

For large-scale recombinant production of bacterial polymers, non-polymer producing bacteria were exposed to biosynthesis pathways. Polymers such as PHA, CGP (cyanophycin granule peptide), HA (hyaluronic acid), and PGA [poly-y-glutamate] were produced by these methods [89, 85-96]. For example, recombinant E.coli [89] was fermented for the lai e-scale production of PHA [89]. In addition the PHB biosynthesis genes of Ralstonia eutropa were harbored in E.coli to produce poljmers such as PHA composed of (R)-S-hydroxybutyrate and (R)-3-hydroxyvalerate and/or (R)-3-hydroxyhexanoate which showed preferable properties for use in industrial applications [97-99, 85-96]. 

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