Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Recognition of MHC Class I-Bound Peptides by TCR

A prerequisite for CTL-mediated immune response is the formation of the MHC-class I-peptide complex and subsequent recognition by the T-cell repertoire, which can be analyzed in cell lysis assays with MCr-loaded target cells. Chromium release is a measure for peptide-induced cell lysis by CTL, and indicates the potency of the peptide to serve as an allele-specific epitope. A synthetic epitope has been identified with the peptide library approach to elucidate the molecular basis for the observed cross-recognition of two ligands by a single receptor [53]. [Pg.360]

Screening of 152 0/X7 peptide libraries on cytolysis of target cells by several CTL clones showed clone-specific Recognition Patterns which allow the deduction of synthetic T-cell epitopes effective in the concentrations also typical for naturally occurring epitopes [54,55]. The degree of degeneracy is characteristic for a certain CTL clone and is also represented in the Recognition Pattern of the peptide library. [Pg.360]

The approach succeeded in the construction of functional mimics of tumor epitopes. One peptide was identified that induces a CTL response in vivo and thus represents the functional mimicries of a CTL epitope. The value of peptide libraries for the identifica- [Pg.360]

Combinatorial peptide libraries, either completely random or characterized by one or several defined positions, are useful tools for the identification of the critical features of MHC class I binding peptides and of natural and synthetic epitopes. The complete Recognition Pattern of an 0/X7 library with 152 individual peptide mixtures represents the influence of amino acid residues on mediating contact to the MHC class I molecules and of residues crucial for recognition by the TCR [54,55]. [Pg.361]

A major hindrance for transplantation arises from the high frequency of alloreactive T cells, although the molecular basis of alloreactivity remains elusive. Recently, a combinatorial peptide library was used to study I-Ep-alloreactivity of a hemoglobin (64-76)/ -Ek- [Pg.361]


See other pages where Recognition of MHC Class I-Bound Peptides by TCR is mentioned: [Pg.360]   


SEARCH



Class I MHC

MHC

MHC class

MHC class I peptide

MHC-peptide

© 2024 chempedia.info