Receptor Activation, Tyrosine Kinase Activity, and in Cultured Vascular Smooth Muscle Cells

Receptor Activation, Tyrosine Kinase Activity, and [Ca +]f in Cultured Vascular Smooth Muscle Cells 

Canine Femoral Vascular Smooth Muscle Cells 

In canine VSMC, pronounced heterogeneity of the cellular Ca + response evoked by receptor activation 

Preincubation of the cells with genistein invariably blocked the Ca + response evoked by either phenylephrine or serotonin (Figs. 4C and 4D). Though not shown, the inhibitory effects of genistein were reversible as replacement of the medium with genistein-free PSS resulted in recovery of responsiveness to both phenylephrine and serotonin. Previous studies showed that the concentration of genistein used in these experiments virtually abolished the tyrosine kinase activity of pp60 = in extracts from smooth muscle and other 

JOSEPH DI SALVO, NIHAL KAPLAN, AND LORI A. SEMENCHUK 

---