Real time monitoring, wave signal

Sapsford etal. (2001) examined microarray-based antibody-antigen binding kinetics in real time to determine the effect of spot size. Capture antibodies were immobilized in an array pattern onto silver-clad microscope slides. Antimouse IgG was directly attached to the surface or attached via neutravidin capture of the biotinylated antibody. Cy5-labeled mouse IgG capture was monitored based upon the signal generated from the excitation of an evanescent wave guide (slide) with a 635-nm laser source detection was achieved by a charge-coupled device (CCD) camera system. Both static and flow-through conditions were employed. 

In a typical procedure, a continuous wave laser (50 mW) excites ICG at 780 nm and the fluorescence signal is captured at 830 nm. Subsequent to a bolus injection in the tail vein of the rat, the time-dependent clearance of ICG from plasma can be monitored in real-time as shown in Eig. 9 for three rat data sets. 

An efficient algorithm is required to monitor the oscillatory wave signal in real time. The flow chart is shown in Figure 6. 

The ion signal I t) is detected as a function of the time delay between pump and probe pulses. As a result the wave packet propagation of interest can be monitored in real time as an oscillation of the ion signal I. The period of the ion signal is related to the classical frequency cjci of the molecular vibration. For example, if the Condon point is at a turning point, one would expect the signal to oscillate at frequency cJci- The signal is... 

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