RAPD-PCR

Randomly amplified polymeric DNA (RAPD) PCR Repetitive extragenic palindromic (REP) PCR BOX PCR... 

Epe, C., Bienioschek, S., Rehbein, S. and Schnieder, T. (1995) Comparative RAPD-PCR analysis of lungworms (Dictyocaulidae) from fallow deer, cattle, sheep and horses. Journal of Veterinary Medicine B 42, 187-191. 

Another popular technique for endophyte identification is RFLP (restriction fragment length polymorphism).In this technique, nuclear or mitochondrial DNA is extracted from the endophyte and digested with restriction endonucleases. The digestion products are separated by gel electrophoresis, and a unique pattern emerges for the fungus, similar to the patterns seen in the RAPD-PCR technique. 

Azab, M.E., Bishara, S.A., Helmy, H., Oteifa, N.M., El-Hoseiny, L.M., Ramzy, R.M. and Ahmed, MA. (2004) Molecular characterization of Egyptian human and animal Echinococcus granulosus isolates by RAPD-PCR technique, journal of the Egyptian Society of Parasitology 34, 83-96. 

Debaryomyces hansenii var. fabryii and its anamorph C. famata var. flareri were reported to be pathogenic by Vazquez et al. [223] and Nicand et al. [224]. Using random amplified polymorphic DNA analysis (RAPD-PCR), Prillinger et al. [208] considered D. hansenii ar. fabryii as a distinct species, i.e. 

Hormonema dematioides is a very similar dimorphic fungus, but can be differentiated from A. pullulans by the absence of synchronous conidiation, by different physiological profiles and genotypic approaches like RAPD-PCR [151]. It is also occasionally pathogenic in humans [262]. 

For the identification of new yeast species from nature we use a new polyphasic approach [452] (1) Species delimitation using RAPD-PCR or... 

AFLP (2) Species identification using partial sequences of the 18S or 26S rDNA and corroboration of the identification data using RAPD-PCR and the respective type strain (3) Ubiquinone system according to Messner et al. [340], and (4) Qualitative and quantitative monosaccharide pattern of purified and hydrolyzed cell walls [449],... 

Messner R, Schweigkofler W, Ibl M, Berg G, Prillinger H Molecular characterization of the plant pathogen Verticillium dahliae Kleb. using RAPD-PCR and sequencing of the 18S rRNA-gene. JPhytopathol 1996 144 347-354. 

Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV DNA-polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 1990 18 6531-6535. Molnar O, Prillinger H, Lopandic K, Weigang F, Staudacher E Analysis of coenzyme Q systems, monosaccharide patterns of purified cell walls, and RAPD-PCR patterns in the genus... 

Molnar O, Messner R, Prillinger H, Scheide K, Stahl U, Silberhumer H, Wunderer W Genotypische Identifiziemng von Saccharomyces-Krton aus der Getrankeindustrie mit Hilfe der Zufallsprimer-abhangigen Polymerase-Kettenreaktion (RAPD-PCR). Mitt Klosterneuburg 1995 45 113-122. 

Fig. 2.3 RAPD-PCR fingerprinting of different wine lactic acid bacteria species (CRA-Istituto Sperimentale per I Enologia)...

Du Plessis, E.M., Dicks, L.M.T. (1995). Evaluation of random amplified polymorphic DNA (RAPD)-PCR as a method to differentiate Lactobacillus acidophilus, Lactobacillus crispatus, Lactobacillus amylovorans, Lactobacillus gallinarum, Lactobacillus gasseri, and Lactobacillus johnsonii. Curr. Microbiol, 31, 114-118. 

Reguant, C., Bordons, A. (2003). Typification of Oenococcus oeni strains by multiplex RAPD-PCR and study of population dynamics during MLF. J. Appl. Microbiol., 95,... 

Rossetti, L., Giraffa, G. (2005). Rapid identification of diary lactic acid bacteria by M-13 generated RAPD-PCR fingerprint database./. Microbiol. Methods, 63, 135-144. 

Fig. 3 UPGMA-based dendrogram obtained by RAPD-PCR with primer M13 for Bacillus cereus (Be) and B. thuringiensis (Bt) strains using 70% similarity...

Andrighetto C, Zampese L, Lombardi A (2001) RAPD-PCR Characterization of Lactobacilli isolated from artisanal meat plants and traditional fermented sausages of Veneto region (Italy). Lett Appl Microbiol 33 26-30... 

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