Monosaccharides patterns

Other characters that generally corroborate the Urediniomycetes are the 5S rRNA secondary structure of type A [236], plate-like spindle pole bodies, the cell wall monosaccharide pattern (table 1) and simple septa tapering towards the pore or poreless septa [22, 217, 234, 335, 336, 340, 343-348]. The members of this class are predominantly dimorphic except the Uredinales... 

T. minor is a member of the Georgefischerales which caused subcutaneous infection in an immunosuppressed patient [361, 375], Based on the qualitative and quantitative monosaccharide pattern of purified cell walls, T. minor fits well in the Ustilaginomycetes [215],... 

AFLP (2) Species identification using partial sequences of the 18S or 26S rDNA and corroboration of the identification data using RAPD-PCR and the respective type strain (3) Ubiquinone system according to Messner et al. [340], and (4) Qualitative and quantitative monosaccharide pattern of purified and hydrolyzed cell walls [449],... 

Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV DNA-polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 1990 18 6531-6535. Molnar O, Prillinger H, Lopandic K, Weigang F, Staudacher E Analysis of coenzyme Q systems, monosaccharide patterns of purified cell walls, and RAPD-PCR patterns in the genus... 

Ethylene dithioacetals and diethyl dithioacetals have been investigated for a number of monosaccharides. The c.d. spectra show one, or two, c.d. band(s) of low intensity between 235 and 250 nm, and a third band of low intensity that peaks below 220 nm. These workers found no overall relationship between the configurational pattern of the monosaccharide and the sign of these bands. However, there does appear to be a correlation between the configurations of C-2, C-3 and C-4 and the sign of the c.d. band that peaks below 220 nm. 

Constitutional studies have broadly followed the pattern of methyla-tion of the unsubstituted alcoholic groups in a carbohydrate, hydrolytic or oxidative disruption of the molecule and identification of the resulting methylated fragments. In this way methyl ethers have been invaluable in the determination of the ring structures of the monosaccharides and in the elucidation of the constitutions of the more complex saccharides. 

Fig. 4.5.4 Identification of mutations in the transferrin protein by neuraminidase treatment. Unusual patterns in the IEF of serum transferrin might lead to pitfalls in CDG diagnostics. These varying patterns are often due to mutations of charged amino acids in the protein backbone of the transferrin molecule, which might lead, for example, to an accumulation of trisialo transferrin bands (lane 3, indicated by a question mark). Further investigations are carried out by cleaving off charged sialic acid monosaccharide moieties from transferrin-linked oligosaccharides by neuraminidase treatment, followed by IEF and transferrin antibody staining. In the case of protein mutations, additional bands below (lane 4) or above (not shown) the desialylated transferrin form appear...

As revealed by the data available, the type of compound closest to the ideal for structural analysis of monosaccharides is the class of dialkyl dithioacetals or their acetates their mass spectra contain a considerable peak due to molecular ion, and their fragmentation patterns are simple enough (due to the absence of a sugar ring) and specific enough to permit determination of the position of substituents on the basis of the position of peaks. Thus, elimination is characteristic of the C-2-substituents, whereas substituents at C-3 tend to be retained, producing a peculiar difference between the mass spectra. However, the mass spectra of dialkyl dithioacetals provide almost no information regarding the stereochemistry of the monosaccharide molecule. 

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