Random amplified polymorphic DNA-PCR

Zapparoli, G., Reguant, C., Bordons, A., Torriani, S., and Dellaglio, F. (2000). Genomic DNA fingerprinting of Oenococcus oeni strains by pulsed-field gel electrophoresis and randomly amplified polymorphic DNA-PCR. Curr. Microbiol. 40,351-355. 

Martin, A., Jurado, M., Rodriguez, M., Nunez, R, and Cordoba, J. J., Characterization of molds from dry cured meat products and their metabolites by micellar electrokinetic capillary electrophoresis and random amplified polymorphic DNA PCR, J. Food Prot., 67, 2234, 2004. 

As described by Williams et al. (1990), random amplified polymorphic DNA-PCR (RAPD-PCR) is a variant of PGR that utilizes oligonucleotide probes (9 to 12 base pairs or bp) to amplify several regions of the genome. The amplification products are then separated electrophoretically. Resolution depends upon the primer sequence and reaction conditions. RAPD-PCR can be made more specific by use of highly specific oligonucleotide probes. Holt and Cote (1998) applied this technique toward the identification of dextran-producing Oenococcus strains, and Esteve-Zarzoso et al. (1998) were able to identify Saccharomyces and Zygosaccharomyces species. Quesada and Cenis (1995) used the method to characterize wine yeasts. 

Wu, Z., Nagano, I. and Takahashi, Y. (1998) The detection of Trichinella with polymerase chain reaction (PCR) primers constructed using sequences of random amplified polymorphic DNA (RAPD) or sequences of complementary DNA encoding excretory-secretory (E-S) glycoproteins. Parasitology 117, 173-183. 

Scanning electron microscopy, light microscopy, and confocal laser scanning microscopy together with FISH Randomly amplified polymorphic DNA (RAPD), Enterobacterial repetitive intergenic consensus sequence (ERIC-PCR)... 

AGE — agarose gel electrophoresis, BAL = bronchoalveolar fluid, FAM = 6-carboxyfluorescein label, MGB = minor groove binder, n.s. - not specified, RAPD = randomly amplified polymorphic DNA, RT-PCR = reverse transcription PCR, TAMRA = 6-carboxytetramethylrhodamine label. 

Debaryomyces hansenii var. fabryii and its anamorph C. famata var. flareri were reported to be pathogenic by Vazquez et al. [223] and Nicand et al. [224]. Using random amplified polymorphic DNA analysis (RAPD-PCR), Prillinger et al. [208] considered D. hansenii ar. fabryii as a distinct species, i.e. 

Du Plessis, E.M., Dicks, L.M.T. (1995). Evaluation of random amplified polymorphic DNA (RAPD)-PCR as a method to differentiate Lactobacillus acidophilus, Lactobacillus crispatus, Lactobacillus amylovorans, Lactobacillus gallinarum, Lactobacillus gasseri, and Lactobacillus johnsonii. Curr. Microbiol, 31, 114-118. 

SSCP Single Stand Conformation Polymorphism RAPD Randomly Amplified Polymorphic DNA rep-PCR RE Restriction Endonclease... 

Three research groups independently developed the method, with small differences between them. Williams et al. (31) patented the RAPD technique (Randomly Amplified Polymorphic DNA), which became the most popular. Welsh and McClelland (32) used primers with 20 nucleotides and called the technique Arbitrary Primed-PCR. Finally, Caetano-Anolles et al. (33) described the same technology with name DNA Amplihcation Fingerprint. 

Coton et al. (2005b) developed a PCR-based amplified ribosomal DNA restriction analysis method for rapid detection of Zymomonas at the subspecies level. A further duplex PCR method with primers specihc for 23S rRNA gene for detection of Zymomonas species has been developed (Coton et al., 2005b). This method could detect Zymomonas species within 24 h with sensitivity of 10 CFU/ml (Coton et al., 2005b). Coton, Laplace, Auffray, and Coton (2006) characterised several strains of Z. mobilis with random amplified polymorphic DNA (Table 8.3). 

Quesada, M.P. andJ.L. Genis. 1995. Use of random amplified polymorphic DNA (RAPD-PCR) in the characterization of wine yeasts. Am. J. Enol. Vitic. 46 ... 

Identification of the species and characterization of the dominant strains in static surface acetic acid fermentation were required for the stabilization of fermentation procedures and improvement of the strain. Recent advances of genetic analyses including DNA—DNA hybridization, enterobacterial repetitive intergenic consensus (ERlC)-polymerase chain reaction (PCR) and repetitive extragenic palindromic (REP)-PCR, random amplified polymorphic DNA (RAPD), and 16S rRNA sequence analysis with PCR-denaturing gradient gel electrophoresis (DGGE) methods allow identificatiOTi of species of acetic acid bacteria. 

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