Micellar electrokinetic capillary electrophoresis

A kit solutions for non-charged molecules based on micellar electrokinetic capillary electrophoresis... 

Thomas, B. R., and Ghodbane, S. (1993). Evaluation of a mixed micellar electrokinetic capillary electrophoresis method for validated pharmaceutical quality-control. /. Liq. Chromatogr. 16, 1983-2006. 

The separation of phospholipids by micellar electrokinetic capillary electrophoresis (MEKC) has been described (17-19). In this technique, solutes are separated based on their distribution between a mobile (usually aqueous) and a pseudostationary (micellar) phase. Szucs et al. found that the major soybean phospholipids were fully resolved in only 7 minutes using deox ycholic acid for micelle formation in combination with 30% n-propanol at 50°C (18). However, quantification of the separated compounds remains troublesome. This is due first of all to the fact that only UV detection can be used, thus making the response highly dependent on the degree of unsaturation of the phospholipids. Besides, the comparison of peak areas in MEKC is more complicated than in HPLC, because all compounds are moving with different velocities. 

Northorp DM, MacCrehan WA (1992) Sample collection, preparation, and quantitation in the micellar electrokinetic capillary electrophoresis of gunshot residues. J Liquid Chromatogr 15 1041-1063. 

Northrop DM, Martire DE, MacCrehan WA (1991) Separation and identification of organic gunshot and explosive constituents by micellar electrokinetic capillary electrophoresis. Anal Chem 63 1038-1042. 

Zhao, Y. and Lunte, C. E. 1997. Determination of caffeine and its metabolites by micellar electrokinetic capillary electrophoresis. J. Chromatogr. B 688 265-74. 

Martin, A., Jurado, M., Rodriguez, M., Nunez, R, and Cordoba, J. J., Characterization of molds from dry cured meat products and their metabolites by micellar electrokinetic capillary electrophoresis and random amplified polymorphic DNA PCR, J. Food Prot., 67, 2234, 2004. 

Artifactual-injection-related peak splitting can occur under certain conditions. When the sample diluent contains organic solvents and micellar electrokinetic capillary electrophoresis or cyclodextrin containing electrolytes are employed, splitting can occur due to the distribution of the solute between two phases moving at different speeds... 

Figure 23-21 Separation of neutral molecules by micellar electrokinetic capillary electrophoresis. The average plate count in this experiment is 250 000 in 50 cm of capillary length. [J. T. Smith. W. Nashabeh, and Z. E. Rassi, Anal. Chem. 1994,66,1119.]...

D. "Gunshot Residue Analysis by Micellar Electrokinetic Capillary Electrophoresis Assessment for ApplicaHon to Casework" (Parts 1 and 2). Journal cf Forensic Sciences 46 (2001), 549-572. 

The pathological condition induced by exposing an organism to a toxic substance depends on the mode of admission, the quantity, and the type of dosage (acute or chronic). Urine profiling by p-cyclodextrin-modified micellar electrokinetic capillary electrophoresis was used by Zomer et al. to identify the type of cadmium intoxication (acute or chronic).Their dataset of 96 samples was split into a learning set of 60 samples and a test set of 36 samples. Discriminant analysis applied to the first six principal components had better results on the test set (96.97% correctly classified) than did SVM trained on the original measured data (75.76% correctly classified). 

Thormartn, W., Meier, R, Marcolli, C., and Binder, F., Analysis of barbiturates in human serum and urine by high-performance capillary electrophoresis-micellar electrokinetic capillary chromatography with on-column multiwavelength detection, /. Chromatogr., 545, 445, 1991. 

High-efficiency separations of FQ-labeled proteins are only achieved in the presence of an anionic surfactant, such as SDS. As a result, capillary isoelectric focusing is not useful for the analysis of these proteins. Instead, we employ capillary sieving electrophoresis and micellar electrokinetic capillary chromatography for our two-dimensional electrophoresis. 

Mallampati, S., Leonard, S., De Vulder, S., Hoogmartens, J., and Van Schepdael, A. (2005). Method development and validation for the analysis of didanosine using micellar electrokinetic capillary chromatography. Electrophoresis 26, 4079-4088. 

Bendahl, L., Hansen, S. H., and Gammelgaard, B. (2001). Capillary modified by noncovalent anionic polymer adsorption for the capillary zone electrophoresis, micellar electrokinetic capillary chromatography and capillary electrophoresis mass spectrometry. Electrophoresis 22, 2565-2573. 

After a short introduction into the relevance of Impurity profiling for regulatory authorities, public health, and the pharmaceutical industry, an overview is presented based on the various modes of capillary electrophoresis that have been used in drug impurity analysis. The applications of capillary zone electrophoresis, non-aqueous capillary electrophoresis, micellar electrokinetic capillary chromatography, microemulsion electrokinetic capillary chromatography, capillary gel electrophoresis, and capillary electrochromatography are presented consecutively. 

Ackermans, M. T., Everaerts, F. M., and Beckers, J. L. (1992). Determination of aminoglycoside antibiotics in pharmaceuticals by capillary zone electrophoresis with indirect UV detection coupled with micellar electrokinetic capillary chromatography.. Chromatogr. 606, 229—235. 

Altria, K. D., and Smith, N. W. (1991). Pharmaceutical analysis by capillary zone electrophoresis and micellar electrokinetic capillary chromatography. /. Chromatogr. 538, 506-509. 

Kang, J. W., De Reymaeker, G., Van Schepdael, A., Roets, E., and Hoogmartens, J. (2001). Analysis of bacitracin by micellar electrokinetic capillary chromatography with mixed micelle in acidic solution. Electrophoresis 22, 1356-1362. 

Wienen, R, and Holzgrabe, U. (2003). A new micellar electrokinetic capillary chromatography method for separation of the components of the aminoglycoside antibiotics. Electrophoresis 24, 2948-2957. 

Trenerry, V. C., Robertson, J., and Wells, R. J. (1994). The determination of cocaine and related substances by micellar electrokinetic capillary chromatography. Electrophoresis 15, 103-108. 

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