Radioautograph

The radioautographic work suggests another model illustrated in Fig. XII-11. The load is supported over area A, with metal contacts of shear strength s over a portion of the area ctA and film-film contacts of shear strength Sf over the rest of the area. In analogy to Eq. XII-9, one can write the total frictional force, F as... 

Rosier, O. Joh, T.H. and Beaudet, A. Ultrastmctural relationships between serotonin and dopamine neurons in the rat arcuate nucleus and medial zona incerta A combined radioautographic and immunocytochemical study. Neurosci Lett 48 279-285, 1984. 

Figures 2 and 3. Peel Section and Radioautograph from Elemental S35 Treatment...

Figures 8 and 9. PeelJSec-tion and Radioautograph from H2S 04 Treatment...

Subheading 5., regarding analyses and interpretation of radioautographs, offers additional information regarding exposure time. 

Construct a radioautographic sandwich consisting of a slide with a thin layer of collodion containing pollen and another slide to which was affixed a thin layer of Kodak NTB-2 Nuclear Track Emulsion (use appropriate safelight and bulb wattage see Table 1). 

Protocol updated from Krolak et al. (49) and Dashek and Mills (50). Controls (a) Germinate pollen without [14C]-pro and process as before, (b) Construct a radioautographic sandwich with collodion lacking pollen, (c) Expose a slide coated with Kodak NTB-2 Nuclear Track Emulsion to light and develop emulsion with D-19. (Sample autoradiographs are presented in Fig. 3.)... 

Fig. 3. Lightmicroscope radioautographs ofintact tubes from pollen germinated 4 h in a medium containing [14C]-proline with or without the proline analogue, azetidine-2-carboxylic acid (AZC). A = 1.0 mM AZC, B = 0.1 mM AZC, C = 0.01 mM AZC, D = 0.001 mM AZC, E = Control. From Dashek and Mills (50), with permission. 

Kopriwa BM. A semiautomatic instrument for the radioautographic coating technique. J Histochem Cytochem 1966 14 923-928. 

Jerry NL. Quantity dipping and processing of radioautographic slides. J Biol Photogr Assoc 1967 35 73-82. 

Belanger LF. Staining processed radioautographs. Stain Technol 1961 36 313-317. 

Stemam U. Loss of silver grains from radioautographs stained by gallocyanin-chrome alum. Stain Technol 1962 37 231-234. 

Both resolution and statistical analyses of EM radioautographs have been discussed in depth by Evans and Callow (3) and Morel (7). Table 2 reveals that reducing section thickness as well as diameters of halide crystals and developed grains improve resolution of EM radioautographs. With regard to statistical analysis, Morel (7) considers the probability-circle method and cross-fire method for the quantitative analysis of EM autoradiographs. 

Fig. 3. Diagram of an EM radioautograph. (From Rogers [20]. with permission.)... 

Effect of Section Thickness Emulsion Type and Development on Resolution of EM Radioautographs... 

Protocol for Preparing Light Microscopic Radioautographs from Resin-Embedded Tissues (Adapted from Morel [7])... 

Coronas V, Krantic S, Jourdan F, Moyse E. 1999. Dopamine receptor coupling to adenylyl cyclase in rat olfactory pathway a combined pharmacological-radioautographic approach. Neuroscience 90 69-78. 

Beaudet, A. Dournaud, P. Boudin, H. Complementarity of radioautographic and immunohistochemical techniques for localizing neuroreceptors at the light and electron microscopy level. Braz. J. Med. Biol. Res. 1998, 37(2), 215-223. 

Stein, 0. and Stein, Y. 1967. Lipid synthesis, intracellular transport, and secretion. II. Electron microscopic radioautographic study of the mouse lactating mammary gland. J. Cell Biol. 34, 251-263. 

The size distribution of radioactive compounds was studied by Leary and Fitzgibbon (38, 39), who used radioautographic techniques. The method is especially useful for solid particles in the size range of 0.1 to 10 microns and may have limited application, as a research tool only, in the study of fuel sprays. 

Figure 12.9. (a) Optical images, (b) radioautographic images, and (c) MALDI-MS/MS images from study rat brain tissue section (Hsieh et al., 2006a). 

In both Southern and Northern analyses restriction-digested DNA fragments, mRNA, and polyA mRNA are separated by size when electrophoresed on agarose gel. The separated molecules are transferred, by electroblotting or capillary blotting, on to a nylon or nitrocellulose membrane. The immobilized RNA or DNA is reacted with a radiolabeled, chemiluminescent, or fluorescent probe that is complementary to the DNA/RNA of interest, unbound probe is washed off, and the membrane exposed, in the case of radioactive probes, to radioautographic film to visualize the sample of interest. 

Bertherat J, Dournaud P, Berod A, Normand E, Bloch B, Rostene W, Kordon K, Epelbaum J (1992) Growth hormone-releasing hormone-synthesizing neurons are a subpopulation of somatostatin receptor-labeled cells in the rat arcuate nucleus a combined in situ hybridization and receptor light microscopic radioautographic study. Neuroendocrinology 56 25-31. 

Moyse, E, Rostene W, Vial M, Leonard K, Mazella J, Kitabgi P, Vincent JP, Beaudet A (1987) Distribution of neurotensin binding sites in rat brain a light microscopic radioautographic study using monoiodo 125I-tyr3-neurotensin. Neuroscience 22 525-536. 

Moeschlin S, Speck B. 1967. Experimental studies on the mechanism of action of benzene on the bone marrow (radioautographic studies using 3H-thymidinc). Acta Haematol 38 104-111. 

Kaplan MS, Hinds JW (1977) Neurogenesis in the adult rat Electron microscopic analysis of light radioautographs. Science 197 1092-1094. 

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