Radioautographic techniques

The size distribution of radioactive compounds was studied by Leary and Fitzgibbon (38, 39), who used radioautographic techniques. The method is especially useful for solid particles in the size range of 0.1 to 10 microns and may have limited application, as a research tool only, in the study of fuel sprays. 

The exact location of the iodine-trapping mechanism within the thyroid gland is not known, but it is generally assumed that the membranes of the epithelial cells of the thyroid follicles are involved. Attempts to solve this important problem have included the use of radioautographic techniques. After administration of radioactive iodine, greater con-... 

Synthetic activity as evidenced by high levels of glycosyltransferases has been observed in Golgi apparatus-rich fractions and radioautographic techniques have shown the incorporation of labeled sugars in the Golgi apparatus of intact cells (Molnar et al., 1969 Ray et al., 1969 Schach-ter et al., 1970 Cheetham et al., 1970 Neutra and Leblond, 1966 Northcote, 1969 Ito, 1969). 

Since B lymphocytes have receptors on their surface, they can selectively bind antigen. Bound antigen can be visualized by means of radioautographic techniques, rosette formation, enzymatic reactions and fluorescent techniques. In this section, I intend to collect some estimates on the frequency of antigenbinding cells from normal non-immunized animals. Because the estimation of the frequencies by these techniques is not based on analyzing the capacity of cells to produce an antibody response, special attention must be paid to the specificity of the binding and its relevance to the antibody response. 

Kopriwa BM. A semiautomatic instrument for the radioautographic coating technique. J Histochem Cytochem 1966 14 923-928. 

Beaudet, A. Dournaud, P. Boudin, H. Complementarity of radioautographic and immunohistochemical techniques for localizing neuroreceptors at the light and electron microscopy level. Braz. J. Med. Biol. Res. 1998, 37(2), 215-223. 

Roth L. J., Diab I M, Watanabe M., and Dinerstein R. (1974) A correlative radioautographic fluorescent and histochemical technique for cytopharmacology. Mol Pharmacol. 10, 986-998. 

Fig. 1. Quantification of Fi in chromatophores from R. rubrum by the Western blotting technique. SDS-polyacrylamide gel electrophoresis (11) was performed with isolated Fj-ATPase from R. rubrum (o) and with chromatophores (0.95 nmol BChl, 1.9 nmol, ). Western blotting was performed as described in ref. 9. A laser scanner (LkB) was used to determine the optical density of the dots for the a- and 3-subunits on the radioautograph.

---