Rat brain tissue section

Figure 12.3. MALDI-MS signals from a droplet deposited in the rat brain tissue section containing a drug candidate are plotted against acquiring time which is directly related to the locations of each pixel.

In a separate experiment, two regions of rat brain tissue sections that contained different levels of clozapine based on the MALDI-IMS results were isolated. The isolated tissue sections were transferred into plastic test tubes and mixed with 300 p,L of 95 5 methanol-water solution for protein precipitation. After centrifugation, a 10-p.L aliquot of the supernatant from each sample was injected for HPLC-MS/MS analysis. The HPLC was operated under a fast gradient condition using a cyano... 

Figure 12.9. (a) Optical images, (b) radioautographic images, and (c) MALDI-MS/MS images from study rat brain tissue section (Hsieh et al., 2006a). 

Wang H, Jackson S, McEuen J, Woods A (2005) Localization and analyses of small drug molecules in rat brain tissue sections. Anal Chem 77(20) 6682-6686. doi 10.1021/ac050868d... 

FIGURE 14.8 Images generated from mass scan data (a) and from MS/MS data (b) for m/z 837 (SPM 24 0) in a rat brain tissue section. The intensity scales are shown at the right of each image. The mass scan data image was normalized to the total ion intensity at each point, while the MS/MS data image is the raw intensity for the transition of m/z % il- m/z 778 (neutral loss, NL, of 59 Da). 

FIGURE 3.21 MALDI-ion mobility 2D plot of a rat brain tissue section with DHB matrix in positive ion detection mode. Many of the peaks in the trend line identified as matrix can be assigned to DHB clusters or DHB clusters -H potassium. Reprinted from Jackson et al. [58]. 

FIGURE 42.22 SIMS Analysis of rat brain tissue sections with different projectiles (E = 20 keV) and sample preparation procedures ( native, no sample pretreatment gold, sputter-coated with 1 nm of Au matrix, deposition of a thin layer of 2,5-DHB with a pneumatic sprayer). Relative intensities of ions are in the range m/z > 600. Figure from Reference [319]. 

Fig. 1.4. Imaging IM-MS for mapping the spatial coordinates of analytes based on structure and molecular weight, (a) Optical Image of a thin coronal rat brain tissue section adjacent the section analyzed by IMS. (b) Lipid imaging of the analytes indicated at an Ion mobility arrival time of 480-484 gs and m/z 700-840 is illustrated in (c)-(e). Extracted ion density maps for two phospholipids at m/z 771-776 and 819-823, respectively. (Reprinted with permission from ref. (59),)...

The methods presented here describe the ability to characterize phosphatidylcholines (PCs), sphingomyelins (SPMs), phos-phatidylserines (PSs), and phosphatidylethanolamines (PEs) in the positive ion mode from rat brain tissue sections using imaging tandem MS and the creation of compound-specific images from full-scan MS and MS, while using MS for the final identification. The use of MS images for the separation of isobaric ions coupled with the ability to perform MS provides the means to identify isobaric species present in the tissue section. 

Fig. 18.4. Example of a typical mass spectrum recorded on a rat brain tissue section after in situ enzymatic digestion of a FFPE or frozen tissue.

Fig. 19.2. (a-b) MALDI-MS spectra recorded on a rat brain tissue section after on tissue trypsin digestion (a) and on a tissue with trypsin digestion followed by derivatization with 3-SBA (b). (c-d) MALDI-MS/MS spectra of one of the peptides generated from digest (m/z 1,339.3) (c) and the same peptide after derivatization with 3-SBA (m/zl, 523.7) (d). 

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