Immunological detection

Immuno localization using polyclonal antibodies raised against AE showed that AE was widely distributed in orange fruit but with more intensive immunological detection in the outer part of the peels e.g. albedo and flavedo and in the segments (juice vesicles). The results indicate that AE is located at the site where the major fraction of pectin is deposited. 

Entrapped drug is shielded from immunological detection and external enzymatic degradation. 

However, Gi2 is unlikely to be the only G protein that couples 8 receptors to adenylyl cyclase since the cloned 8 receptor expressed in HEK 293 cells effectively couples to adenylyl cyclase via a pertussis toxin sensitive G protein [74], yet these cells lack immunologically detectable Gi2 and G0 [78] suggesting that either Gix or Gi3 in these cells couples the 8 receptor to adenylyl cyclase. Prather et al. [69] and Law and Reisine [73] have reported that 8 receptors can associate with Gix and Gi3. Furthermore, Sanchez-Blazquez et al. [68] have reported that antisense against Gi3 mRNA administered to the nervous system blocked 8 agonist-induced analgesia. 

Garlic Immunological detection of a Gar V-type virus Helguera et al. (9)... 

Helguera MA. Immunological detection of a GarV-type vims in Argentine garlic cultivars. Plant Dis 1997 81 1005-1010. 

Flurkey WH. Polyphenoloxidase in higher plants immunological detection and analysis of in vitro translation products. Plant Physiol 1986 86 614-618. 

Craig, O.E. and Collins, M.J. (2000). An improved method for the immunological detection of mineral bound protein using hydrofluoric acid and direct capture. Journal of Immunological Methods 236 89-97. 

Edwards, S. W., Hughes, V., Barlow, J., Bucknall, R. (1988). Immunological detection of myeloperoxidase in synovial fluids from patients with rheumatoid arthritis. Biochem. J. 250, 81-5. 

No studies were located regarding in-depth immunological effects in humans after inhalation exposure to chloroform. Only one study (Bomski et al. 1967) described the health effects of a group of 68 workers occupationally exposed to chloroform for 1-4 years in a pharmaceutical plant. Chloroform air concentrations ranged from 0.01 to 1 mg/L, and other solvents were also reported in the air in trace amounts. Splenomegaly was the only immunologically detected health effect in a small percentage of these cases. 

The Peroxidase-Antiperoxidase (PAP) Method and Other All-Immunologic Detection Methods ... 

Dako DuoCISH Kit contains HRP- and AP-based immunological detection reagents for Texas Red- and FITC-labeled probes. This is an ideal kit for manual dual-color BISH assay detection. 

Peptides complementary to the surface of Ab-2 (which acts as a receptor bioisostere) are selected from a peptide library (Lam et al., 1991 Houghten et al., 1991) using molecular immunology detection methods. Such peptides are expected to be receptor antagonists or agonists (Scott and Smith, 1990). 

Albin, R. Weber, R. Silverman, P.M. The Cpx proteins of Escherichia coli K12. Immunologic detection of the chromosomal cpxA gene product. J. Biol. Chem., 261, 4698-4705 (1986)... 

N. Recombinant rat nucleoside diphosphate kinase isoforms (a and ) purification, properties and application to immunological detection of native isoforms in rat tissues. Biochim. Biophys. Acta, 1205, 113-122 (1994)... 

Western blotting has become an important, modern technique for analysis and characterization of proteins. The procedure consists of, first, the electrophoretic transfer (blotting) of proteins from polyacrylamide gels to synthetic membranes. The transferred blots are then probed using immunological detection methods to identify proteins of specific structure and/or function. In this experiment, bovine serum will be fractionated by SDS-PAGE and the proteins blotted onto a nitrocellulose membrane. Serum glycoproteins will be identified by their specific interaction with the lectin concanavalin A. 

Haselbeck, A. and Hosel, W. (1990) Description and application of an immunological detection system for analyzing glycoproteins on blots. Glycoconjugate J. 7, 63-74. 

Waller H, Friess E, Kiefer J (1981) On the immunological detection of X-ray induced DNA damage. Radiat Environ Biophys 19 259-264... 

Lendaro, E., Ippoliti, R., Bellelli, A., Brunori, M Zito, R Citro, G., et al. (1991). Brief communication On the problem of immunological detection of antigens in skeletal remains. Am. J. Phys. Anthropol. 86, 429-432. 

---