Rabbit pancreas

Poly(A)-rich mRNA prepared from rabbit pancreas directed the synthesis of a protein (mol. wt. 5.65 X 10 ), which, using a cell-free protein synthesizing system derived from wheat embryo, appeared to represent the product of o-amylase mRNA cell-free translation.This protein, which was 1500 daltons larger than the purified rabbit pancreas a-amylase, was resolved by isoelectric focusing into discrete species similar to those observed for purified a-amylase. The protein, which on peptide analysis demonstrated considerable identity with the a-amylase, was selectively precipitated by glycogen, but with a much lower recovery than that observed for rabbit on porcine pancreatic a-amylases. 

Tjalve, H., and Popov, D., 1973, Effect of nicotine and nicotine metabolites on insulin secretion from rabbit pancreas pieces. Endocrinology 92 1343. 

Fig. 2u Insulin secretion in vitro". Insulin release from pieces of rabbit pancreas effected by glucose before and after addition of guinea pig antiinsulin serum. Note complete inhibition of I LA by anti bodies.

Fig. 3. Insulin secretion in vitro" Effect of d-Xylose on insulin secretion in vitro (pieces of rabbit pancreas).

As shown by Telib (1967) in our laboratory, stimulation of insulin secretion in pieces of rabbit pancreas resulted in similar increases following glucose, fructose, ribose and d-xylose, the most effective concentrations of the hexoses and pentoses being 2 mg/ml (Fig.3 as example). No effect was seen following both galactose and d- and 1-arabinose (Fig. 4 as example). 

The positive action of ribose and xylose incubated with pieces of rabbit pancreas is in agreement with the "in vivo" studies of a number of workers (Pozza et al (195s), Biermann et al (1959), lUilliamsl 1966)) but at variance with Grodsky s (1963) observation on the perfused rat pancreas, and the studies of Coore and Randle (1964) who used the incubated rabbit pancreas likewise. 

Regarding stimulation of insulin secretion "in vitro" by amino acids, pieces of rat and rabbit pancreas mere incubated in media consisting of the usual buffer plus o.l, l.o and 10 mg amino acid/ml for 2 and 3 hours, respectively (Pfeiffer et al (1967), Yeboah (1967)). In Fig. 7 are shomn the responses of rabbit and rat pancreas "in vitro" to 1-leucine, the first amino acid shomn to induce insulin secretion "in vivo" in insulinoma patients (Cochrane, et al (1956)). Increases in Iffll concentrations up to 300% of the initial values recorded as 100 mere noted. Higher sensitivity of the rabbit than of the rat 0—... 

Pancreozymins, obtained from "Karolinska Institute", Stockholm, was effective in concentrations of 1,5 and 10 u/ml on pieces of rabbit pancreas (Fig.12). Pancreozymins, obtained from "Boots", England, in concentrations of 1 U/ml, produced increases in insulin output similar to those obtained by the same concentration of pancreozyraine "Karolinska".Following incubation with 5 U of the British preparation/... 

Gastrin Pentapeptide ICI" 1-20 y/i Insulin from Rabbit Pancreas... 

Gastrin-pentapeptide from Imperial Chemical Industries, England, though somewhat less potent clearly induced release of insulin by the rabbit pancreas "in vitro", too (Fig.13). Wore details on the insulin stimulating action of these intestinal hormones are given elsewhere (Schroder et al (1967)). 

Finally, serotonin was also shown to effect insulin release from the rabbit pancreas in the "in vitro" preparation (Fig.14), whereas "in vivo" neither in animals nor in men did stimulation of insulin secretion occur. However, with the exception of this... 

Fig. 15. Insulin secretion in vitro" Insulin release from pieces of rat and rabbit pancreas, respectively, effected by Hb 419 and Tolbutamide in different concentrations. Note the approximately 100 and 1000 times more effective actions of Hb 419 on rat and rabbit pancreas, respectively.

Coore G. and Randle P.3. Regulation of insulin secretion studied with pieces of rabbit pancreas. Biochem. 3. 93, 65, (1964)... 

Jos V, Connolly J, Deardon D et al. A simple method for islet isolation from the rabbit pancreas. Transplantation. 1994 58 390-392. 

Abstract. The hybrid cell lines, which we have obtained, can be widely used in veterinary virology and biotechnology for preparing vaccines, test-systems for viruses. Any strains of hybrid cells are producing the biological active proteins (enzymes and others). We have obtained hybrid cell lines (PO-TKxCO, PO-TKxHO), which are sensitive to prion protein, and also hybrid culture with P-cells of the pancreas of rabbit. 

Parallel activation of Na+/H+ antiport and CI7HCO3 antiport Erythrocytes from dog, rabbit, and Amphiuma lymphocytes osteoclasts endothelial cells parotis pancreas, liver, gallbladder, proximale tubule, medullary thick ascending limb, and collecting duct MDCK cells Activation of Na+-K+-2CI cotransport... 

---