Purification strategy for

The purification strategy for CBPs is conceptually simple. Proteins from carotenoid-rich tissues are separated under nondenaturing and relatively aqueous conditions where carotenoids are expected to remain bound to the CBPs. CBPs are then detected by the color of the carotenoids. 

Spider silk proteins from plants remain soluble at high temperatures, allowing them to be enriched by boiling [26]. In order to enrich the spider silk-ELP fusion protein, we therefore exposed tobacco leaf extracts to heat treatment at 95°C for 60 min and then cleared the supernatant by centrifugation. In further steps, the reversible precipitation behavior of ELP fusion proteins was exploited to develop a suitable purification strategy. For the selective precipitation of SOl-lOOxELP, NaCl was added at a final concentration of 2 M and the temperature was increased to 60 °C. In this man-... 

Another extension of this theoretical smdy is the consideration of both an economical and an effective purification strategy for the 4-RDM. The need for such a purification scheme is motivated by the need to have an N- and 5-representable 4-RDM if one wishes to solve the fourth-order modified contracted Schrodinger equation [62, 64, 87]. There have already been several attemps to purify both the 3-RDM and 4-RDM [18, 34, 52]. In particular, a set of inequalities that bound the diagonal and off-diagonal elements of these high-order matrices have been reported [18]. However, the results obtained with this approach within the framework of the fourth-order modified contracted Schrodinger equation (and the second-order contracted Schrodinger equation) were not fully satisfactory because the different spin-blocks of the matrices did not appear to be properly balanced [87, 114]. 

A purification strategy for any protein at any scale of operation can be broadly divided into three sequential stages—capture, intermediate purification, and polishing. Each stage represents a set of specific problems that may be encountered during a purification process. The nature of the sample and the scale of operation will dictate what equipment and methodology are appropriate to solve the problem. Many of the procedures discussed here can be found in Coligan et al. (2001). 

Saxena, R.K., Sheoran, A., Giri, B., and Davidson, W.S. 2003. Purification strategies for microbial lipases. Journal of Microbiological Methods, 52 1-18. 

D. T. Mclachlin and B. T. Chait, Improved P-eUmination-based affinity purification strategy for enrichment of phosphopeptides, A aZ. Chem. 75 (2003), 6826-6836. 

In this chapter, an overview is given of various purification strategies for automated solution-phase chemistry that have appeared in the recent literature. 

Interest in producing large quantities of supercoiled plasmid DNA has increased as a result of gene therapy and DNA vaccines. Due to the commercial interests in these approaches, the development of production and purification strategies for gene therapy vectors has been performed in pharmaceutical companies within a confidential environment. It is thus important to describe the downstream operations for the large-scale purification of plasmid DNA to attain a final product that meets specifications and safety requuements. " ... 

Figure 2.9 Purification strategy for the polyhydroxyalkanoates. The recovery of polyhydroxyalkanoates is composed of three steps pre-treatment, extraction and purification.

Figure 8.6 Solid-phase-based purification strategy for DNA-templated synthesis. Reprinted with permission from Professor David R. Liu.

While the overall purification strategy for the recombinant mammalian Secl3/31 complex is relatively straightforward, there are a few caveats that... 

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