Pseudomonas saccharophila

Chen S-W, MD Aitken (1999) Salicylate stimulates the degradation of high-molecular weight polycyclic aromatic hydrocarbons by Pseudomonas saccharophila P15. Environ Sci Technol 33 435-439. 

Thus, D-fructose can be synthesized by a reversal of the Embden-Meyer-hof glycolytic pathway (see Fig. 2) in animals and in many other organisms. Unless evidence is available to the contrary, it is generally assumed that this pathway is present in any organism, since distribution studies indicate the system is ubiquitous. However, a different pathway is used by Pseudomonas saccharophila, which utilizes one mole of D-glucose or D-gluconic acid... 

The enzyme has been found in Leuconostoc mesenteroides66 and Pseudomonas saccharophila.6 In a series of outstanding researches Doudoroff and Hassid with associates investigated the specificity requirements of sucrose phosphorylase from P. saccharophila. It was found that the enzyme exhibits an absolute specificity for the D-glucose moiety of its... 

If PAH-degrading microorganisms use broad-specificity enzymes or common pathways to transform multiple PAHs, then inducers for the metabolism of one PAH substrate might co-induce the transformation of a range of PAHs. Preliminary evidence indicated that the transformation of naphthalene, phenanthrene, fluoranthene, and pyrene by Pseudomonas saccharophila P15 was stimulated by salicylate [132], a known inducer of naphthalene metabolism in pseudomonads [43]. However, Chen and Aitken [181] reported in more detail the inducing effects of salicylate on the transformation of various HMW PAHs by Pseudomonas saccharophila P15 isolated from contaminated soil, including... 

In general, the study suggested that Pseudomonas saccharophila P15 expressed a low level of constitutive PAH metabolism which was inducible to much higher levels and that HMW PAH metabolism by this microorganism was induced by the low-molecular weight substrates phenanthrene and salicylate. 

The first description of a bacterial FDPase was that of Fossitt and Bernstein (89), who purified the enzyme from extracts of Pseudomonas saccharophila and established the specificity of the enzyme and the stoichiometry of the reaction. Fructosediphosphatase has also been reported in Aerobacter aerogenes (90), where the enzyme is required for growth on D-fructose. Like the enzyme in E. coli, the Aerobacter FDPase exhibits optimum activity between pH 7 and 8. In this organism the obligatory pathway for fructose utilization is fructose - fructose 1-phosphate -> fructose 1,6-diphosphate. The presence of FDPase is required as a source of fructose 6-phosphate for biosynthetic pathways. 

Although sucrose has not been synthesized by strictly chemical means, its synthesis has been accomplished by the use of enzymes from living organisms.86 An enzyme from the bacterium Pseudomonas saccharophila Doudoroff was allowed to act on D-glucose-l-phosphate in the presence of D-fructose. This synthesis gives little information about... 

Crystalline alpha-amylases have been prepared from hog pancreas, human pancreas and saliva, " rat pancreas, malted barley, Aspergillus oryzae, - - Pseudomonas saccharophila, Bacillus subtilis, °- Bacillus coagulans, and Bacillus stearothermoph-ilus, whilst extensively purified enzymes have been obtained from malted sorghum, soya beans,broad beans, malted wheat, pigeon pancreas, and shore crab. The preparative procedures usually involve precipitations by ammonium sulfate and acetone. 

An example is the reaction catalyzed by sucrose phosphorylase from Pseudomonas saccharophila ... 

Doudoroff, M. 1940. The oxidative assimilation of sugars and related substances by Pseudomonas saccharophila with a contribution to the direct respiration of di- and poly-saccharides. Enzymologia 9 59-72. 

Another mechanism for the enzymic synthesis of saccharides is one that occurs through transfer of the glycosyl group from a glycosyl phosphate to an appropriate acceptor. An example of such a reaction is the synthesis of sucrose by the enzyme, sucrose phosphorylase, found in Pseudomonas saccharophila ... 

Entner N, Doudoroff M (1952) Glucose and Gluconic Acid Oxidation of Pseudomonas saccharophila. J Biol Chem 196 853... 

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