Phosphorylase Pseudomonas saccharophila

The enzyme has been found in Leuconostoc mesenteroides66 and Pseudomonas saccharophila.6 In a series of outstanding researches Doudoroff and Hassid with associates investigated the specificity requirements of sucrose phosphorylase from P. saccharophila. It was found that the enzyme exhibits an absolute specificity for the D-glucose moiety of its... 

An example is the reaction catalyzed by sucrose phosphorylase from Pseudomonas saccharophila ... 

Another mechanism for the enzymic synthesis of saccharides is one that occurs through transfer of the glycosyl group from a glycosyl phosphate to an appropriate acceptor. An example of such a reaction is the synthesis of sucrose by the enzyme, sucrose phosphorylase, found in Pseudomonas saccharophila ... 

C H2oO,o 312.273 Synth, by the action of Pseudomonas saccharophila phosphorylase on a-glueose 1-phosphate plus L-arabinose. Dihydrate. [a]i3 +156 (c, 2.0 in H2O). 

The in vitro enzymatic synthesis of sucrose was accomplished by Hassid, Doudoroff, and Barker 100), They found that a bacterial preparation from Pseudomonas saccharophila contains a phosphorylase capable of catalyzing the reversible reaction ... 

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