Recombinant Pseudomonas putida

Pseudomonas putida, recombinant PolyOHB-co-3HV-co-4HV- co-3HHX-CO-3HO) Octanoic acid + levulinic add 20 43 Steinbiichel and Gorenflo (1997)... 

Galan Sicilia, B., et al., Method for Desulfurization of Dibenzothiophene Using a Recombinant Pseudomonas Putida Strain as Biocatalyst Patent No. W00170996. 2002, June 16. 

Noda, K. Watanabe, K., and Maruhashi, K., Recombinant Pseudomonas Putida Carrying Both the Dsz and Hcu Genes Can Desulfurize Dibenzothiophene in n-Tetradecane. Biotechnology Letters, 2003. 25(14) pp. 1147-1150. 

The naphtho[2,3-d]-l,3-dioxole was oxidized using recombinant E. coli whole cells overexpressing the gene for the naphthalene dioxygenase from Pseudomonas putida G7. The linear carbohydrate fragment was enzymatically formed after ozon-ization of the diol, by chain extension with a dihydroxyacetone fragment in an... 

K. Miyamotoa, K. Okuroa, H. Ohta, Substrate specificity and reaction mechanism of recombinant styrene oxide isomerase from Pseudomonas putida. Tetrahedron Lett. 48 (2007) 3255. 

Diniz, C.S., Voss, I., and Steinbuchel, A. (2006) Optimization of cyanophycin production in recombinant strains of Pseudomonas putida and Ralstonia eutropha employing elementary mode analysis and statistical experimental design. Biotechnol. Bioeng., 93,... 

In a selection strategy based on the substrate 1-phenyl-n-propylamine (PPA) as the sole source of nitrogen in a chemostat, a recombinant Pseudomonas putida strain carrying the R-transaminase gene, a single amino acid change, Y112F, presumably at or near the active site, improved enantioselectivity of the reaction of racemic... 

The c/s-dihydroxylation reaction catalyzed by these dioxygenases is typically highly enantioselective (often >98% ee) and, as a result, has proven particularly useful as a source of chiral synthetic intermediates (2,4). Chiral cis-dihydrodiols have been made available commercially and a practical laboratory procedure for the oxidation of chlorobenzene to IS, 2S)-3-chlorocyclohexa-3,5-diene-l,2-c diol by a mutant strain of Pseudomonas putida has been published (6). Transformation with whole cells can be achieved either by mutant strains that lack the second enzyme in the aromatic catabolic pathway, cw-dihydrodiol dehydrogenase (E.C. 1.3.1.19), or by recombinant strains expressing the cloned dioxygenase. This biocatalytic process is scalable, and has been used to synthesize polymer precursors such as 3-hydroxyphenylacetylene, an intermediate in the production of acetylene-terminated resins (7). A synthesis of polyphenylene was developed by ICI whereby ftie product of enzymatic benzene dioxygenation, c/s-cyclohexa-3,5-diene-1,2-diol, was acetylated and polymerized as shown in Scheme 2 (8). 

Lei Y., Mulchandani R, Chen W., and Mulchandani A., Direct determination of p-nitrophenyl substituent organophosphorus nerve agents using a recombinant Pseudomonas putida JS444-modified dark oxygen electrode, J. Agric. Food Chem., 53, 524-527, 2005. 

J., and Urlacher, V.B. (2011) Biosynthesis of zeaxanthin in recombinant Pseudomonas putida. Appl. Microbiol. Biotechnol, 89 (4), 1137-1147. 

Domrose, A., Klein, A.S., Hage-Hiilsmaim, J., Thies, S., Svensson, V, Classen, T., Pietruszka, J., Jaeger, K.E., Drepper, T., and Loeschcke, A. (2015) Efficient recombinant production of prodigiosin in Pseudomonas putida. Front. Microbiol, 6, 972. 

Dammeyer, X, Steinwand, M., Kruger, S.C., Diibel, S., Must, M., and Timmis, K.N. (2011) Efficient production of soluble recombinant single chain Ev fragments by a Pseudomonas putida strain KT2440 cell factory. Microh. Cell Fact, 10, 11. 

Qiu, Y.-Z., Han, J., Guo, J.-J., Chen, G.-Q., Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from gluconate and glucose by recombinant Aeromonas hydrophlla and Pseudomonas putida. Biotechnoloov hpttp.rs. 18, 1381-1386 (2005), DOl 10.1007/sl0529-005-3685-6. 

Bioconversion of Toluene to p-Hydroxybenzoate Using a Recombinant Pseudomonas putida... 

In another study by Dennis and co-workers [41], the PHA accumulation of several microorganisms Escherichia coli, Klebsiella aerogenes and PHA-negative mutants of Cupriavidus necator and Pseudomonas putida) that expressed the phaC and acetoacetyl-coenzyme A reductase phaB) of Cupriavidus necator were analysed. This results in a construct that puts phaC and phaB under the control of the original Cupriavidus necator promoter. It was found that wild-type Cupriavidus necator was able to produce sc/-PHA, however the recombinant Cupriavidus necator expressing its own phaC and phaB without P-ketothiolase phaA) were able to accumulate poly(3HB-co-3HHx) when cultivated with even number chain fatty acids. The same trend was reported for Klebsiella aerogenes and Pseudomonas putida. 

Ren Q, de Roo G, Beilen JB, Zinn M, Kessler B, Witholt B (2005b) Poly(3-hydroxyalkanoate) polymerase synthesis and in vitro activity in recombinant Escherichia coli and Pseudomonas putida. Appl Microbiol Biotechnol 286 286-292 Ren Q, Grubelnik A, Hoerler M, Ruth K, Hartmann R, Felber H, Zinn M (2005c) Bacterial poly(hydroxyalkanoates) as a source of chiral hydroxyalkanoic acids. Biomacromolecules 6 2290-2298... 

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