Pseudomonas Jluorescens

Williams employed complexes of Al, Rh, or Ir in combination with Pseudomonas Jluorescens lipase (PFL) for the DKR of 1-phenylethanol. The best results were obtained using Rh2(OAc)4 as the catalyst for the racemization, and 60% conversion of the alcohol to give 1-phenylethyl acetate in 98% ee was obtained (Figure 4.6) [19]. At higher conversion, the enantiomeric excess dropped and 76% conversion gave 80% ee. 

Aliphatic open-chain monooxygenase (AOCMO) Pseudomonas Jluorescens DSM 50106 2006 [139] 2006 [139] [139]... 

Although a maj ority of research activities were dedicated to cycloketone converting BVMOs, the recently discovered novel MOs also enable stereoselective oxidation of noncyclic ketones to esters. An aliphatic open-chain monooxygenase (AOCMO) from Pseudomonas Jluorescens DSM 50106 displays stereoselective biooxidation of terminal acyl-groups in proximity to hydroxyls (Scheme 9.23). The biooxidation gives acetic... 

More recently, the degradation of a-pinene by Pseudomonas jluorescens NCIMB 11671 was described [97,98]. A novel pathway for the microbial breakdown of a-pinene (119) was proposed, Fig. (23). The attack is initiated by enzymatic oxygenation of the 1,2-double bond to form the epoxide (127). This epoxide then undergoes rapid rearrangement to produce a novel diunsaturated aldehyde, occurring as two isomeric forms. The primary product of the reaction (Z)-2-methyl-5-isopropylhexa-2,5-dien-l-al (trivial name isonovalal) (128) can undergo chemical isomerisation to the -form (novalal) (129). Isonovalal, the native form of... 

Also ( Rj-fl- Lys 4 is found to be a substructure of several antibiotics. Examples are the hydrazinodipeptide negamycin 26 from Streptomyces purpeofuscus, bellenamine 28 from Streptomyces nashvillensis (Actinobacteria), and sperabillins A-D 25 from Pseudomonas Jluorescens (Proteobacteria). 

Pseudomonas Jluorescens 200 11.5- 16 Devoid of various reductase and dehydrogenase activities tested 1... 

A phenotypically similar type of resistance is observed following the colonization of plant roots by selected strains of non-padiogenic Pseudomonas Jluorescens bacteria. This type of Induced resistance is called rhizobacteria-mediated induced systemic resistance (ISR) [7]. 

PhiHppot, L., Mirleau, P., Mazurier, S., Siblot, S., Hartmann, A., Lemanceau, P., et al. (2001). Characterization and transcriptional analysis of Pseudomonas Jluorescens denitrifying clusters... 

While it is a powerful solvent, DMSO is undesirable for industrial use. Instead, the surfactant Triton X-100 can be an effective replacement for DMSO in enzymatic hydrolyses [14], and in our case, worked very well. Triton X-100 forms micelles when mixed with water and would solubilize low concentrations of ester for hydrolysis. However, increasing the surfactant charge did not accelerate hydrolysis. In an attempt to reduce the somewhat long reaction time, we screened the enzymes Amano PS Lipase XIII, Pseudomonas jluorescens and Candida cylindracea versus Amano PS-30. PS-30 was still considered the best choice of enzyme when... 

Copolymerization of 8-VL with e-CL using lipase from Pseudomonas Jluorescens, and copolymerization of 8-OL with e-CL and DDL using immobilized form of CALB, were reported by Kobayashi and coworkers [80]. In this later report, copolymerization was performed in isooctane at 60 °C for 48 h, and, according to 13C NMR analysis, random-structured copolymers were obtained with Mn values up to 9000 and yields up to 97%. 

HLL Humicula lanuginosa lipase PFL Pseudomonas jluorescens (later renamed Pseudomonas cepacia) lipase PPL porcine pancreas lipase PSL Pseudomonas cepacia lipase RJL Rhizopusjaponicus lipase. 

All of the above syntheses resulted in racemic 2-methoxy acids, but the stereospecific synthesis of the naturally occurring (R)-2-methoxyhexadecanoic acid was also recently reported [35]. This synthesis started with commercially available ( )-2-hydroxyhexadecanoic acid for which the S enantiomer was selectively acetylated (47 % yield out of a maximum 50%, > 95% ee) with vinyl acetate in THF using the lipase Pseudomonas Jluorescens from Aldrich, Fig. (14). In order to facilitate the separation of the acetylated acid from the non-acetylated acid, both compounds were methylated with diazomethane, thus avoiding cleavage of the acetate functionality. The methyl (R)-2-... 

If an inducer is particularly expensive or not readily available, it may be possible to substitute a compound which can be converted by the organism to the required inducer. For example, in Pseudomonas Jluorescens, kynurenin induces kynureninase the same effect can be obtained by adding tryptophan, a precursor of kynurenin, to the medium (Hayaishi and Stanier, 1951). 

Lockwood LB, Stodola FH. Preliminary studies on the production of a-ketoglutaric acid by Pseudomonas Jluorescens.J Biol Ghem 1946 164 81-3. 

Continuous-flow mode asymmetric acetylation of the prochiral diol 16b with isopropenyl acetate was performed in a small stainless steel PBR filled with sol-gel/Celite entrapped Pseudomonas Jluorescens lipase (PfL) (Lipase AK) [107]. Optimization of the process resulted in (R)-17b with up to 91% ee. 

Kiziak, C., Conradt, D., Stolz, A., Mattes, R., and Mein, J. (2005) Nitrilase from Pseudomonas jluorescens EBC191 cloning and heterologous expression of the gene and biochemical characterization of the recombinant enzyme. Microbiology, 151, 3639-3648. 

Durham (1956) isolated p-hydroxybenzoio acid as an intermediate from a Pseudomonas jluorescens that could utilize PABA as sole substrate. The steps from p-hydroxybenzoic acid to aliphatic compounds have been traced for pseudomonads in the well-known studies of Stanier and his co-workers. 

Another exanple of such non-conventional, innovative methods is applying immunoglobulin solutions films on the surface of carbon steel and stainless steel that has been shown to prevent the adherence of Pseudomonas jluorescens on these metallic surfaces, thus inhibiting biofilm formation [4]. However, this method would not become popular in industry, perhaps because of reasons such as... 

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