Pseudomonas growth

A fermentation such as that of Pseudomonas dentrificans typicaby requires 3—6 days. A submerged culture is employed with glucose, comsteep Hquor and/or yeast extract, and a cobalt source (nitrate or chloride). Other minerals may be required for optimal growth. pH control at 6—7 is usuaby required and is achieved by ammonium or calcium salts. Under most conditions, adequate 5,6-dimethylben2imida2ole is produced in the fermentation. However, in some circumstances, supplementation maybe required. 

Although essential amino acids are requited by both host and tumor, deprivation of select essential amino acids for 2—3 weeks is tolerated by the host yet exerts a pronounced antiproliferative effect on the tumor. Thus, treatment of mice with indole-3-alkane-a-hydroxylase [63363-76-8] from Pseudomonas, which transforms L-tryptophan [73-22-3] to 3-indolylglycaldehyde, lowers the concentration of L-tryptophan in plasma, brain, and lungs, and inhibits the growth of a variety of tumors (32—34). 

Tricyclic pyrazole derivatives (698) are described by Hashem et al. as inhibitors of the growth of Bacillus subtilis, Pseudomonas fluorescens, Staphylococcus aureus and KB cells at moderate concentrations (76JMC229). 

Rai and Constantinides [14] developed a mathematieal model for the fermentation of the baeterium Pseudomonas ovalis, whieh eonverts the glueose to glueonie aeid. The following equations deseribe the dynamies of the logarithmie growth phase ... 

The first step in the complete biodegradation of primary alcohol sulfates seems to be the hydrolysis to yield alcohol. Sulfatases are able to hydrolyze primary alcohol sulfates. Different authors have isolated and used several sulfia-tase enzymes belonging to Pseudomonas species. The alcohol obtained as a result of the hydrolysis, provided that dehydrogenases have been removed to avoid the oxidation of the alcohol, was identified by chromatography and other methods [388-394]. The absence of oxygen uptake in the splitting of different primary alcohol sulfates also confirms the hydrolysis instead of oxidation [395, 396]. The hydrolysis may acidify the medium and stop the bacterial growth in the absence of pH control [397-399]. 

Fig. 4. a) Inhibition of microbial growth by GA, from Bacillus subtilis. Micrococcus luteus and Pseudomonas aeruginosa, b) Growth curve of Bacillus subtilis at 32 ° C ( ) Milk, (O) milk with addedGA47pM. 

It is known that NHase used in industry has a lower optimum temperature," therefore many reports have been concerned with screening for thermostable NHase. Miyanaga et al. has succeeded to analyze the X-ray structure of such a thermostable NHase from Pseudomonas thermophila. Bacillus sp. BR449 producing NHase with optimum temperature of 55°C has been isolated. Similarly, Bacillus sp. RAPc8 has a growth optimum at 65°C. Takashima et al. has isolated Bacillus smithii strain SC-J05-1, with optimum temperature at 40°C, and whose NHase has an optimum temperature and pH of 50°C and 10, respectively. Its crystal structure has also been elucidated. Bacillus pallidus strain Dac521 has... 

A strain of Escherichia coli produces a naphthotriazole from 2,3-diaminonaphthalene and nitrite that is formed from nitrate by the action of nitrate reductase. The initial product is NO, which is converted by reactions with oxygen into the active nitrosylating agent that reacts chemically with the amine (Ji and Hollocher 1988). A comparable reaction may plausibly account for the formation of dimethylnitrosamine by Pseudomonas stutzeri during growth with dimethylamine in the presence of nitrite (Mills and Alexander 1976) (Figure 2.2f). 

Engesser KH, E Schmidt, H-J Knackmuss (1980) Adaptation of Alcaligenes eutrophus B9 and Pseudomonas sp. B13 to 2-fluorobenzoate as growth substrate. Appl Environ Microbiol 39 68-73. 

Hartig C, N Loffhagen, H Harms (2005) Formation of trans fatty acids is not involved in growth-linked membrane adaptation of Pseudomonas putida. Appl Environ Microbiol 71 1915-1922. 

One of the organisms fulfills the need for a growth requirement by the other, for example, vitamin requirements of one organism that is provided by the other. Examples are provided by biotin in cocultures of Methylocystis sp. and Xanthobacter sp. (Lidstrom-O Connor et al. 1983), and thiamin in cocultures of Pseudomonas aeruginosa and an undefined Pseudomonas sp. that degraded the phosphonate herbicide glyphosate (Moore et al. 1983). 

In contrast, cells of Pseudomonas acidovorans strain M3GY during growth with biphenyl have been shown to degrade DDE with the fission of one ring and production of 4-chlorobenzoate (Hay and Eocht 1998). 

Ahn 1-S, WC Ghiorse, LW Lion, ML Shuler (1998) Growth kinetics of Pseudomonas putida G7 on naphthalene and toxicity during nutrient deprivation. Biotechnol Bioeng 59 587-594. 

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