Pseudomonas acidovorans

FIGURE 3.12 Biodegradation of 3,4-dihydroxybenzoate mediated by (a) 4,5-dioxygenase in Pseudomonas acidovorans and (b) 2,3-dioxygenase in Bacillus macerans. 

Harpel MR, JD Lipscomb (1990) Gentisate 1,2-dioxygenase fiomPseudontonas. Purification, characterization, and comparison of the enzymers from Pseudomonas testosteroni and Pseudomonas acidovorans. J Biol Chem 265 6301-6311. 

In contrast, cells of Pseudomonas acidovorans strain M3GY during growth with biphenyl have been shown to degrade DDE with the fission of one ring and production of 4-chlorobenzoate (Hay and Eocht 1998). 

Robert-Gero M, M Poiret, RY Stanier (1969) The function of the beta-ketoadipate pathway in Pseudomonas acidovorans. J Gen Microbiol 57 207-214. 

Hay AG, DD Focht (1998) Cometabolism of l,l-dichloro-2-2-bis(4-chlorophenyl)ethylene by Pseudomonas acidovorans M3GY grown on biphenyl. Appl Environ Microbiol 64 2141-2146. 

Gentisate is formed by a strain of Bacillus sp. in an unusual rearrangement from 4-hydroxybenzoate (Crawford 1976) that is formally analogous to the formation of 2,5-dihydroxyphenylacetate from 4-hydroxyphenylacetate by Pseudomonas acidovorans (Hareland et al. 1975). Similarly, the metabolism of 4-hydroxybenzoate by the archaeon Haloarcula sp. strain D1 involves the formation of 2,5-dihydroxybenzoate (Fairley et al. 2002). All these reactions putatively involve an NIH shift. 

Hareland WA, RL Crawford, PJ Chapman, S Dagley (1975) Metabolic function and properties of 4-hydroxy-phenylacetic acid 1-hydrolase from Pseudomonas acidovorans. J Bacteriol 121 272-285. 

Sparnins VL, S Dagley (1975) Alternative routes of aromatic catabolism in Pseudomonas acidovorans and Pseudomonas putida gallic acid as a substrate and inhibitor of dioxygenases. J Bacteriol 124 1374-1381. 

Loidl M, C Hinteregger, G Ditzelmtiller, A Ferschl, F Streichsbier (1990) Degradation of aniline and mono-chlorinated anilines by soil-borne Pseudomonas acidovorans stmins. Arch Microbiol 155 56-61. 

Sulfur may also be extracted from organic molecules and then support replication, as indicated by utilization of 0,0-diethylphosphorothioate and 0,0-diethylphosphoro-dithioate as sulfur sources by Pseudomonas acidovorans. 

McCullar, M. V., Brenner, V., Adams, R. H. Focht, D. D. (1994). Construction of a novel polychlorinated biphenyl-degrading bacterium utilization of 3,4 -dichlorobiphenyl by Pseudomonas acidovorans M3GY. Applied and Environmental Microbiology, 60, 3833—9-... 

Pyrazinecarboxylic acid underwent microbiological hydroxylation to give 3-oxo-3,4-dihydro- (36, R = H) (Alcaligenes eutrophus 70%), 5-oxo-4, 5-dihydro- (37) (.Pseudomonas acidovorans 96%), or 6-oxo-l,6-dihydro-2-pyrazinecarboxylic acid (38) (Alcaligenes faecalis 85%) 1091 Similar procedures afforded 5-chloro-3-oxo-3,4-dihydro-2-pyrazinecarboxylic acid (Alcaligenes eutrophus 50%) and 5-oxo-4,5-dihydro-2-pyrazinecarbonitrile (Agrobacterium sp 78%).1091... 

Biodegradation degradation c, = 2.0 d by microorganisms Pseudomonas acidovorans 256-1) from soil or waste water at 30°C (Kurane et al. 1977). 

Kurane, R., Suzuki, T., Takahara, Y. (1977) Isolation of microorganisms growing on phthalate esters and degradation of phthalate ester by Pseudomonas acidovorans 256-1. Agric. Biol. Chem. 41, 2119-2123. 

Zhang L., Kuniyoshi L, Hirai M., and Shoda M. (1991) Oxidation of dimethylsulfide by Pseudomonas acidovorans DMR-11 isolated from a peat biofilter. Biotechol. Lett. 13, 223-228. 

Benzoic acid Alcaligenes eutophus Aspergillus niger Azotobacter sp. Bacillus sp. Pseudomonas sp. Pseudomonas acidovorans Pseudomonas testosteroni Pseudomonas sp. strain HI Pseudomonas PN-1... 

In studying the biochemical genetics of tryptophan synthesis in Pseudomonas acidovorans, Buvinger et al.6 isolated 60 independent tryptophan auxotrophs of Pseudomonas acidovorans and characterized them for nutritional response to intermediates of the pathway, accumulation of intermediates, and levels of tryptophan-synthetic enzymes. Mutants for each of the seven proteins catalyzing the five steps of tryptophan synthesis were... 

---