Pseudomonas aeruginosa purification

Abdelala, A.T. Bibb, W.R Nainan, O. Carbamate kinase from Pseudomonas aeruginosa purification, characterization, physiological role, and regulation. J. Bacteriol., 151, 1411-1419 (1982)... 

Haas, D. Leisinger, T. N-acetylglutamate 5-phosphotransferase of Pseudomonas aeruginosa. Purification and ligand-directed association-dissociation. Eur. J. Biochem., 52, 365-375 (1975)... 

Entsch B, DP Ballou (1989) Purification, properties, and oxygen reactivity of /)-hydroxybenzoate hydroxylase from Pseudomonas aeruginosa. Biochim Biophys Acta 999 253-260. 

Furukawa K, N Arimura (1987) Purification and properties of 2,3-dihydroxybiphenyl dioxygenase from polychlorinated biphenyl-degrading Pseudomonas pseudoalcaligenes and Pseudomonas aeruginosa carrying the cloned bphC gene. J Bacterial 169 924-927. 

Wang CL, You SL, Wang SL (2006) Purification and characterization of a novel catechol 1, 2-dioxygenase from Pseudomonas aeruginosa with benzoic acid as a carbon source. Process Biochem 41 1594—1601... 

Okino, N., Tani, M., Imayama, S., and Ito, M., 1998, Purification and characterization ofa novel ceramidase from Pseudomonas aeruginosa. J. Biol. Chem. 273 14368-14373. 

Gudat, J. C., Singh, J., and Wharton, D. C. (1973). Cytochrome oxidase from Pseudomonas aeruginosa I. Purification and some properties. Biochim. Biophys. Acta 292, 376-390. 

Parr, S. R., Barber, D., Greenwood, C., Phillips, B. W., and Melling, J. (1976). A purification ptocedure for the soluble cytochrome oxidase and some other respiratory proteins from Pseudomonas aeruginosa. Biochem. J. 157, 423-430. 

Krishna, R.V. Leisinger, T. Biosynthesis of proline in Pseudomonas aeruginosa. Partial purification and characterization of y-glutamyl kinase. Biochem. J., 181, 215-222 (1979)... 

R. J. Siehnel, C. Egli and R. E. Hancock (1992). Polyphosphate-selective porin OprO of Pseudomonas aeruginosa expression, purification and sequence. Mol. Microbiol., 6, 2319-2326. 

Pseudomonas aeruginosa nitrite reductase of, 274, 275 transhydrogenase of, 53 molecular properties, 57 purification, 54, 56... 

Fig. 10. Purification of a recombinant protein Pseudomonas aeruginosa exotoxin A -capture step.

T2. Tani, Y., Nagasawa, T., Sugisaki, H., and Ogata, K., Microbiol cholinesterase. 11. Purification and characterization of the cholinesterase of Pseudomona aeruginosa. Agric. Biol Chem. 39, 1287-1294 (1975). 

Qi, Q., Steinbtichel, A., and Rehm, B.H.A. (2000) In vitro synthesis of poly(3 -hydroxydecanoate) purification of type II polyhydroxyalkanoate synthases PhaCl and PhaC2 from Pseudomonas aeruginosa and development of an enzyme assay. Appl. Microbiol. Biotechnol., 54, 37-43. 

The purification of a 2-aminoethylphosphonate aminotransferase from Pseudomonas aeruginosa should allow the catabolism of 2-aminoethylphosphonic acid (17) to be studied in detail. This enzyme, which requires (9) as cofactor, catalyses the conversion of (17) to phosphonoacetaldehyde (18). The next enzyme in the catabolic pathway which catalyses the breakdown of (18) should be of general interest as few enzymes are known which can cleave a C-P bond. 

Mitsuhashi and coworkers found streptomycin phosphate transferase in Pseudomonas aeruginosa TI-13 they isolated the inactivated compound, which was shown to have the empirical formula of a mono-0-phosphonodihydrostreptomycin monocarbonate trihydrate by elementary analysis. They also found the same enzyme in other strains, namely, 99, 137, 138, 351, 10126, Cape 18, and TI-11. The enzyme in the supernatant liquor (600 ml) from centrifugation of disrupted eells of strain TI-13 at 105,000g was precipitated by ammonium sulfate at 33-66% saturation. Further purification by successive, column chromatography on Sephadex G-75, DEAE-Sephadex A-50 (with a gradient of potassium... 

Brown, S. M., Howell, M L., Vasil, M. B., Anderson, A. J., and Hassett, D. J. (1995). Cloning and characterization of the katB gene of Pseudomonas aeruginosa encoding a hydrogen peroxide-indudble catalase Purification of KatB, cellular localization, and demonstration that it is essential for optimal resistance to hydrogen peroxide. /. Bacteriol. 177,6536-6544. 

Sharon C, Furugoh S, Yamakido T et al. (1998) Purification and characterization of a lipase from Pseudomonas aeruginosa KKA-5 and its role in ctistor oil hydrolysis. J Ind Microbiol Biotech-nol 20 304-307... 

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