Streptomycin phosphate transferase

Mitsuhashi and coworkers found streptomycin phosphate transferase in Pseudomonas aeruginosa TI-13 they isolated the inactivated compound, which was shown to have the empirical formula of a mono-0-phosphonodihydrostreptomycin monocarbonate trihydrate by elementary analysis. They also found the same enzyme in other strains, namely, 99, 137, 138, 351, 10126, Cape 18, and TI-11. The enzyme in the supernatant liquor (600 ml) from centrifugation of disrupted eells of strain TI-13 at 105,000g was precipitated by ammonium sulfate at 33-66% saturation. Further purification by successive, column chromatography on Sephadex G-75, DEAE-Sephadex A-50 (with a gradient of potassium... 

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