Prussian blue-based glucose biosensor

FIGURE 13.8 Principal scheme of the Prussian blue-based glucose biosensor. 

A. Karaykin, O. Gitelmacher, and E. Karaykina, Prussian blue-based first-generation biosensor. A sensitive amperometric electrode for glucose. Anal. Chem. 67, 2419—2423 (1995). 

Combined with the attractive performance of a Prussian blue-based hydrogen peroxide transducer, the proposed immobilization protocol provides elaboration of the most advantageous first-generation glucose biosensor concerning its sensitivity and detection limit. 

M. Pravda, M.P. O Halloran, M.P. Kreuzer and G.G. Guilbault, Composite glucose biosensor based on screen-printed electrodes bulk modified with Prussian blue and glucose oxidase, Anal. Lett., 35 (2002) 959-970. 

Tan, X.C.,Tian, Y.X., Cai, P.X. andZou, X.Y. (2005) Glucose biosensor based on glucose oxidase immobilized in sol—gel chitosan/silica hybrid composite film on Prussian blue modified glass carbon electrode. Analytical and Bioanalytical Chemistry, 381, 500-507. 

Metal hexacyanoferrates-based biosensors were developed for analysis of glucose [11, 114, 118, 127, 147, 149, 152, 155-166], ethanol [11], D-alanine [147], oxalate [167-169], cholesterol [170, 171], glutamate [114, 119], sucrose [172], and choline [163], Among the transducers used Prussian blue undoubtedly dominates especially if one takes into account that instead of both chromium and cobalt hexacyanoferrates the activity of the transducers in publications [149, 159, 167, 168] was most probably provided by Prussian blue [117]. The sensitivity of cupric hexacyanoferrate is several orders of magnitude lower compared to Prussian blue. However, chemically synthesized... 

A.A. Karyakin, O.V. Gitelmacher, and E.E. Karyakina, A high-sensitive glucose amperometric biosensor based on Prussian blue modified electrodes. Anal. Lett. 11, 2861—2869 (1994). 

Q. Deng, B. Li, and S. Dong, Self-gelatinizable copolymer immobilized glucose biosensor based on Prussian Blue modified graphite electrode. Analyst 123, 1995-1999 (1998). 

R. Garjonyte and A. Malinauskas, Amperometric glucose biosensors based on Prussian blue and polyaniline glucose oxidase modified electrodes, Biosens. Bioelectron., 15 (2000) 445-451. 

R. Garjonyte and A. Malinauskas, Glucose biosensor based on glucose oxidase immobilized in electropolymerized polypyrrole and poly(o-phe-nylenediammine) films on a Prussian blue-modified electrode, Sens. Actuators B, 63 (2000) 122-128. 

The system was first applied for development of chemosensors for gaseous hydrogen chloride. Polyaniline, and its copolymers with different derivates of aniline were used. Then a similar approach was tested in the author s group for optimization of amperometric biosensors for glucose based on electrocatalytical detection of hydrogen peroxide. A pigment Prussian blue was used as an electrocatalyst for decomposition of this product of enzymatic oxidation of... 

---