First-generation glucose biosensors

FIGURE 6-4 Schematic of a first-generation glucose biosensor (based on a probe manufactured by YSI Inc.). 

Combined with the attractive performance of a Prussian blue-based hydrogen peroxide transducer, the proposed immobilization protocol provides elaboration of the most advantageous first-generation glucose biosensor concerning its sensitivity and detection limit. 

A. Karaykin, O. Gitelmacher, and E. Karaykina, Prussian blue-based first-generation biosensor. A sensitive amperometric electrode for glucose. Anal. Chem. 67, 2419—2423 (1995). 

A.A. Karyakin, E.A. Kotel nikova, L.V. Lukachova, E.E. Karyakina, and J. Wang, Optimal environment for glucose oxidase in perfluorosulfonated ionomer membranes improvement of first-generation biosensors. Anal. Chem. 74, 1597—1603 (2002). 

Amperometric glucose biosensors based on co-immobiUzation of GOx with [Os(bpy)2(4-VP)ioCl]Cl in electrochemically generated polyphenol film [72]. A bilayer electrode in which Os-pol3nmer is first adsorbed on a Pt electrode and then electrochemically deposited on polyphenol-GOx fihn showed sensitivity of 1.63-1.79 /jA/cm /mM to 20 mM glucose at 0.4 V and 6-7 mM. Low background current and less interference by common electroactive compounds were noted. 

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