Proteomic analysis laser capture microdissection

Ornstein DK et al. Proteomic analysis of laser capture microdissected human prostate cancer and in vitro prostate cell lines. Electrophoresis 2000 21 2235-2242. 

The sample materials from which proteins for proteomics studies may be extracted include fresh or snap-frozen cells from varied sources such as biological fluids, (serum, urine, plasma) and solid tissues such as biopsy specimens. Moreover, proteins isolated from ethanol-fixed paraffin-embedded tissues can be utilized for MS analysis.2 Protocols for the identification of proteins from formalin-fixed paraffin-embedded (FFPE) tissues have been recently developed.3 4 FFPE materials are the most common forms of biopsy archives utilized worldwide, and represent an important advancement for the large-scale interrogation of proteins in archival patient-derived materials. Finally, laser capture microdissected tissues have been successfully used for MS analysis.45... 

Paweletz CP, Liotta LA, Petricorn EE (2001) New technologies for biomarker analysis of prostate cancer progression laser capture microdissection and tissue proteomics. Urology 57 160-163... 

VanMeter AJ, Rodriguez AS, Bowman ED et al (2008) Laser capture microdissection and protein microarray analysis of human non-smaU cell lung cancer differential epidermal growth factor receptor (EGPR) phosphorylation events associated with mutated EGFR compared with wild type. Mol Cell Proteomics 7 1902-1924... 

Banks BE, Dunn MJ, Eorbes MA, et al. (1999) The potential use of laser capture microdissection to selectively obtain distinct populations of ceUs for proteomic analysis - preliminary findings. Electrophoresis 20, 689-700. 

Li C, Hong Y, Tan YX et al. Accurate qualitative and quantitative proteomic analysis of clinical hepatocellular carcinoma using laser capture microdissection coupled with isotope-coded affinity tag and two-dimensional liquid chromatography mass spectrometry. Mo/ Cell Proteomics 2004,3399-409. 

In 2001, both Lawrie et al. and Moskaluk coupled LCM (laser capture microdissection) with 2D gel proteomics to recover proteins from laser captured micro-dissected tissue in a form that can be used in 2D gel analysis and mass spectrometry. This may provide valuable information for protein profiling and databasing of human tissues in healthy and disease states. 

Mouledous L, Hunt S, Harcourt R, Harry J, Williams KL, Gutstein HB. Navigated laser capture microdissection as an alternative to direct histological staining for proteomic analysis of brain samples. Proteomics 2003 3(5) 610-615. 

Use of laser capture microdissection to selectively obtain distinct populations of cells for proteomic analysis. Methods Enzymol. 356, 33—49. 

Mouledous, L., Hunt, S., Harcourt, R., Harry, J.L., Williams, K.L. and Gutstein, H.B. (2003) Proteomic analysis of immunostained, laser-capture microdissected brain samples. Electrophoresis 24,296-302. Nagy, A. and Delgado-Escueta, A.V. (1984) Rapid preparation of synaptosomes from mammalian brain using nontoxic isoosmotic gradient material (Percoll). J. Neurochem. 43, 1114-1123. 

Paweletz, C.P Liotta, L.A. Petricoin, E.F. New Technologies for Biomarker Analysis of Prostate Cancer Progression Laser Capture Microdissection and Tissue Proteomics, Urology 57,160-163 (2001). 

Cowherd, S.M., Espina, V.A., Petricoin, E.F., 3rd, and Liotta, L.A. (2004) Proteomic analysis of human breast cancer tissue with laser-capture microdissection and reverse-phase protein microarrays. Clin Breast Cancer 5, 385-92. 

Zang, L., Palmer Toy, D., Hancock, W.S., Sgroi, D.C., and Karger, B.L. (2004) Proteomic analysis of ductal carcinoma of the breast using laser capture microdissection, LC-MS, and 160/180 isotopic labeling. J Proteome Res 3, 604-12. 

Banks, R., et al. (1999). The Potential Use of Laser Capture Microdissection to Selectively Obtain Distinct Populations of Cells for Proteomic Analysis Preliminary Findings, Electrophoresis 20 689-700. 

Baker, H., Patel, V., Mohnolo, A.A., ShiHitoe, E.J., Ensley, J.F., Yoo, G.H., Meneses-Garcia, A., Myers, J.N., El-Na ar, A.K., Gutkind, J.S., and Hancock, W.S. (2005) Proteome-wide analysis of head and neck squamous cell carcinomas using laser-capture microdissection and tandem mass spectrometry. Oral Oncol, 41,183-199. 

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