Proteins human urine

Bence-Jones protein (human urine) Cryoimmunoglobulin (Glo) 20 0.736 61H1... 

Urokinase (from human urine) [9039-53-6] Mr 53,000, [EC 3.4.21.31]. Crystn of this enzyme is induced at pH 5.0 to 5.3 (4") by careful addition of NaCl with gentle stirring until the soln becomes turbid (silky sheen). The NaCl concentration is increased gradually (over several days) until 98% of saturation is achieved whereby the urokinase crystallises as colourless thin brittle plates. It can be similarly recrystd to maximum specific activity [104K CTA units/mg of protein (Sherry et al. J Lab Clin Med 64 145 1964)]. [Lesuk et al. Science 147 880 1965 NMR Bogusky et al. Biochemistry 28 6728 1989.] It is a plasminogen activator [Gold et al. Biochem J 262 1989 ]. 

Although estrone and estradiol (26) have both been isolated from human urine, it has recently been shown that it is the latter that is the active compound that binds to the so-called estrogen receptor protein. Reduction of estrone with any of a large number of reducing agents (for example, any of the complex metal hydrides) leads cleanly to estradiol. This high degree of stereoselectivity to afford the product of attack at the alpha side of the molecule is characteristic of many reactions of steroids. 

The availability of a cDNA for erythropoietin has made it possible to produce substantial amounts of this hormone for analysis and for therapeutic purposes previously the isolation of erythropoietin from human urine yielded very small amounts of the protein. The major use of recombinant erythropoietin has been in the treatment of a small number of anemic states, such as that due to renal failure. 

Results from experiments using the protocol above27 have shown that anti-Salmonella immunomagnetic beads could be used to unambiguously determine the presence of Salmonella choleraesuis from suspensions of bacterial mixtures. This target organism was also positively identified from spiked samples of river water, human urine, chicken blood, and 1 % milk. For the river water and urine samples, no cross-reactivity was observed and only protein... 

Menotrophin (human menopausal gonadotrophin) is the name given to FSH-enriched extracts from human urine. Such preparations contain variable levels of FH activity, as well as various other proteins normally present in urine. As much as 2.51 of urine may be required to produce one dose (75 IU, -7.5 mg) of human FSH (hFSH). 

A3. Albanese, A. A., Davis, V. I., Smetak, E. M., Lein, M., and Fisher, M., Amino acid analysis of the nondiffusible fraction of enzymatic protein digests and human urine. Arch. Biochem. Biophys. 20, 47-54 (1949). 

Peptides in Human Urine (Skarzynski and Samecka-Keller), 5, 107 Protein Bound Iodine (Chaney), I, 82 Radioactive Iodine-131 in the Diagnosis of Hyperthyroidism, Blood Plasma Levels of (Silver), 1, 111 Transaminase Activities of Serum and Body Fluids, the Clinical Significance of Alterations in (Wroblew-ski), 1, 314... 

FRET to occur, and thus no FI emission is observed. The specificity of this assay was also examined for mixed samples. The mixed lysozyme samples were prepared in fetal bovine serum (FBS), human saliva and human urine. It was found that FAM emission was still visible upon addition of each mixed sample, implying that this assay has a great potential for the detection of real biological samples. This study illuminates that introduction of specific aptamer/protein interaction as the recognition event, and utilization of FRET as the signal transduction channel, is an effective way to develop CPE-based protein sensors with good specificity. 

Tyrosine (Tyr or Y) (4-hydroxyphenylalanine ((5)-2-amino-3-(4-hydroxyphenyl)-propanoic acid)) is a polar, neutral, aromatic amino acid with the formula H00CCH(NH2)CH2C6H50H and is the precursor of thyroxin, dopamine, norepinephrine (noradrenaline), epinephrine (adrenaline), and the pigment melanin. Being the precursor amino acid for the thyroid gland hormone thyroxin, a defect in this may result in hypothyroidism. Tyr is extremely soluble in water, a property that has proven useful in isolating this amino acid from protein hydrolysates. The occurrence of tyrosine- 0-sulfate as a constituent of human urine and fibrinogen has been reported. ... 

Interest in the nature of interactions between shortchain organic surfactants and large molecular weight macromolecules and ions with hydroxyapatite extends to several fields. In the area of carles prevention and control, surfactant adsorption plays an important role in the Initial states of plaque formation (1-5) and in the adhesion of tooth restorative materials ( ). Interaction of hydroxyapatite with polypeptides in human urine is important in human biology as hydroxyapatite has been found as a major or minor component in a majority of kidney stones ( 7). Hydroxyapatite is used in column chromatography as a material for separating proteins (8-9). The flotation separation of apatite from... 

Oligosaccharides with blood group activity are found in human urine (7) and in human milk (3). Materials with blood group activity are widely distributed in nature (1, 2, 3, 4, 5, 6, 7, 8, 9) as are various proteins, plant (19, 20), and animal hemagglutinins, (21, 22, 23) which show specificity for blood group determinants. 

Problems of traceability of total protein and catecholamine determinations in human urine... 

The Czech Reference Material CZ 6007a for total protein and creatinine in human urine was prepared [2], and served as a preliminary batch for the preparation of a certified reference material (CRM) for the stress indicators adrenaline (A), noradrenaline (NA) and dopamine(DA)in human urine. Some major problems in the traceability of these different analytes are presented. 

Several kinds of traceability problems occurred during the preparation of CRM CZ 6007a Total Protein and Creatinine in Human Urine. 

A reference material (RM) for traceability of total protein in human urine does not exist. The control materials used in clinical laboratories for calibration purposes are not unified. Some laboratories use bovine serum albumin as a calibration material (URINE-CHIMIE BIOTROL) [5], whereas others use a mixture of human serum albumin (70%) and globulin (30%) (LYPHOCHECK Quantitative Urine Control, BIO-RAD) [6]. The use of various protein calibration standards yields various results of... 

Table 1 Six methods for the determination of total protein concentration in human urine were compared the Lowry method, biuret method, methods using the dyes Commassie Brilliant Blue (G250), Ponceau-S, pyrogallol red and the turbidity method by Exton...

The values of total protein and A concentrations in human urine are method-dependent. It is necessary to... 

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