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Proteins electrospray ionization mass spectrometry

Samalikova, M. Grandori, R. Role of opposite charges in protein electrospray ionization mass spectrometry. J. Mass Spectrom. 2003, 38, 941-947. [Pg.531]

Electrospray ionization mass spectrometry (ESI-MS) is an analytical method for mass determination of ionized molecules. It is a commonly used method for soft ionization of peptides and proteins in quadmpole, ion-trap, or time-of-flight mass spectrometers. The ionization is performed by application of a high voltage to a stream of liquid emitted from a capillaty. The highly charged droplets are shrunk and the resulting peptide or protein ions are sampled and separated by the mass spectrometer. [Pg.458]

Grandori, R. Origin of the conformation dependence of protein charge-state distributions in electrospray ionization mass spectrometry. I. Mass Spectrom. 2003,38, 11-15. [Pg.252]

Tang, Q., Harrata, A. K., Lee, C. S. (1997). Two-dimensional analysis of recombinant E. coli proteins using capillary isoelectric focusing electrospray ionization mass spectrometry. Anal. Chem. 69(16), 3177-3182. [Pg.241]

Blonder, J., Rodriguez-Galan, M.C., Chan, K.C., Lucas, D.A., Yu, L.R., Conrads, T.P, Issaq, H. J., Young, H.A., Veenstra, T.D. (2004). Analysis of murine natural killer cell microsomal proteins using two-dimensional liquid chromatography coupled to tandem electrospray ionization mass spectrometry. J. Proteome Res. 3, 862-870. [Pg.255]

Huber, C.G., Premstaller, A. (1999). Evaluation of volatile eluents and electrolytes for high-performance liquid chromatography-electrospray ionization mass spectrometry and capillary electrophoresis-electrospray ionization mass spectrometry of proteins. I. Liquid chromatography. J. Chromatogr. A 849, 161-173. [Pg.316]

Whitelegge, J.P, Zhang, H., Aguilera, R., Taylor, R.M., Cramer, W.A. (2002). Full subunit coverage liquid chromatography electrospray ionization mass spectrometry (LCMS+) of an oligomeric membrane protein cytochrome b(6)f complex from spinach and the cyanobacterium Mastigocladus laminosus. Mol. Cell. Proteomics. 1, 816-827. [Pg.317]

Janini, G.M., Zhou, M., Yu, L.-R., Blonder, J., Gignac, M., Comads, T.P., Issaq, H.J., Veenstra, T.D. (2003). On-column sample enrichment for capillary electrophoresis sheathless electrospray ionization mass spectrometry evaluation for peptide analysis and protein identification. Anal. Chem. 75, 5984—5993. [Pg.382]

SMITH, R.D., LOO, J.A, OGORZALEK-LOO, R.R., BUSMAN, M., UDSETH, H.R., Principles and practice of electrospray ionization-mass spectrometry for large polypeptides and proteins, Mass Spec. Rev., 1991,31,472-485. [Pg.58]

Affinity capture-release electrospray ionization mass spectrometry (ACESIMS) is another recently introduced technique for quantification of proteins, and to date has most often been applied to clinical enzymology.60 The product conjugates of the enzymatic reaction between the synthetic substrate and targeted enzyme are captured by immobilized affinity reagents, purified, released into solution, and analyzed by ESI-MS. [Pg.88]

L. Konermann and D. J. Douglas. Unfolding of Proteins Monitored by Electrospray Ionization Mass Spectrometry A Comparison of Positive and Negative Ion Modes. J. Am. Soc. Mass Spectrom., 9(1998) 1248-1254. [Pg.75]

K.L.Johnson, T.D. Veenstra,J.M. Londowski, A.J.Tomlinson, R. Kumar, S. Naylor, On-line sample clean-up and chromatography coupled with electrospray ionization mass spectrometry to characterize the primary sequence and disulfide bond content of recombinant calcium binding proteins, Biomedical Chromatography 13 (1999)37-45. [Pg.6]

The ability to prepare monoliths within a mold of any shape was used by Lee et al. [128] who prepared monolithic ST-DVB microbeads within pulled fused silica needles and used them for the reversed-phase separation and on-line electrospray ionization mass spectrometry (ESI-MS) detection of proteins and peptides. As illustrated by Fig. 18, these monolithic microcolumns separated proteins far better than capillaries packed with commercial C18 silica or polymeric beads. [Pg.115]

Green B.N., Hutton T., and Vinogradov S.N. (1996), Analysis of complex protein and glycoprotein mixtures by electrospray ionization mass spectrometry with maximum entropy processing, Method. Mol. Biol. 61, 279-294. [Pg.271]

Moini M. 2004. Capillary electrophoresis-electrospray ionization mass spectrometry of amino acids, peptides and proteins. Methods Mol Biol 276 253. [Pg.173]

Liang, Z., Duan, J., Zhang, L., Zhang, W., Zhang, Y., and Yan, C. (2004). Pressurized electrochromatography coupled with electrospray ionization mass spectrometry for analysis of peptides and proteins. Anal. Chem. 76, 6935-6940. [Pg.474]

Fion, N., Gellon, J. O., Jensen, H., and Girault, H. H. (2003). On-chip protein sample desalting and preparation for direct coupling with electrospray ionization mass spectrometry. ]. Chromatogr. A 1003, 11-19. [Pg.517]

Figeys, D., and Aebersold, R. (1998). Nanoflow solvent gradient delivery from a microfabricated device for protein identifications by electrospray ionization mass spectrometry. Anal. Chem. 70, 3721-3727. [Pg.520]

Petty, I., Wisniewski, J.R., and Szewczuk, Z. (2001) Conformational stability of six truncated cHMGla proteins studied in their mixture by H/D exchange and electrospray ionization mass spectrometry. Acta Biochim. Pol. 48, 1131-1136. [Pg.128]

Electrospray ionization mass spectrometry (ESI-MS) is a powerful technique for analyzing non-covalent complexes formed between small molecules and proteins. [Pg.65]

J. A. Loo Studying noncovalent protein complexes by electrospray ionization mass spectrometry. Mass Spectrom. Rev. 1997, 16, 1-23. [Pg.117]

Katta, V., Chait, B. T. Conformational-changes in proteins probed by hydrogen-exchange electrospray-ionization mass-spectrometry. Rapid Commun Mass Spectrom 1991, 5, 214-217. [Pg.336]

Ehring H. Hydrogen exchange/ electrospray ionization mass spectrometry studies of structural features of proteins and protein/ protein interactions. Anal. Biochem. 1999, 267, 252-259. [Pg.396]

Wang Y., Gross M.L. Application of hydrogen exchange and electrospray ionization mass spectrometry in studies of protein structure and dynamics, in Applied Electrospray Mass Spectrometry (Practical Spectroscopy), ed. Pramanik BN, Ganguly AK, Gross ML 2002, pp 389-410. [Pg.396]

Ficarro, S. B., Salomon, A. R., BriU, L. M., Mason, D. E., Stettler-GiU, M., Brock, A., and Peters, E. C., Automated immobilized metal affinity chromatography/nano-liquid chromatography/electrospray ionization mass spectrometry platform for profiling protein phosphorylation sites, Rapid Communications in Mass Spectrometry 19(1), 57-71, 2005. [Pg.97]

Wendt, H., Durr, E., Thomas, R. M., Przybylski, M., and Bosshard, H. R. (1995). Characterization of leucine zipper complexes by electrospray ionization mass spectrometry. Protein ScL, 4, 1563-70. [Pg.298]

Yan, J. X. Wait, R. Berkelman, T. Harry, R. A. Westbrook, J. A. Wheeler, . H. Dunn, M. J. A modified silver staining protocol for visualization of proteins compatible with matrix-assisted laser desorption/ionization and electrospray ionization-mass spectrometry. Electrophoresis 2000, 21(11), 3666-3672. [Pg.426]

Affinity capture-release electrospray ionization mass spectrometry (ACESIMS) and isotope-coded affinity tags (ICAT) are two recently introduced techniques for the quantitation of protein activity and content with applications to clinical enzymology... [Pg.152]

R. Bakhtiar, R. Hofstadler, and R. D. Smith, Electrospray Ionization Mass Spectrometry Characterization of Peptides and Proteins, J. Chem. Ed. 1996,... [Pg.679]

Tjernberg, A., et al., Determination of dissociation constants for protein-ligand complexes by electrospray ionization mass spectrometry. Anal Chem, 2004, 76, 4325-4331. [Pg.95]


See other pages where Proteins electrospray ionization mass spectrometry is mentioned: [Pg.1234]    [Pg.296]    [Pg.114]    [Pg.17]    [Pg.333]    [Pg.612]    [Pg.335]    [Pg.345]    [Pg.372]    [Pg.208]    [Pg.97]    [Pg.851]    [Pg.324]    [Pg.102]    [Pg.131]    [Pg.459]   
See also in sourсe #XX -- [ Pg.162 ]




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