Proteins adsorption on polymers

Kim JH, Yoon JY (2002) Protein adsorption on polymer particles. In Hubbard A (ed) Encyclopedia of surface and colloid science. Marcel Dekker, New York... 

Zemla J, Lekka M, Raczkowska J, Bemasik A, Rysz J, Budkowski A. Selective protein adsorption on polymer patterns formed by self-organization and soft lithography. Biomacromolecules 2009 10 2101-9. 

Fundamentals of Native Plasma Protein Adsorption on Polymer Surfaces... 

THE RELATIONSHIP BETWEEN m vivo PROTEIN ADSORPTION ON POLYMER SURFACES AND PLATELET ADHESION... 

Proteins may be covalently attached to the latex particle by a reaction of the chloromethyl group with a-amino groups of lysine residues. We studied this process (17) using bovine serum albumin as a model protein - the reaction is of considerable interest because latex-bound antigens or antibodies may be used for highly sensitive immunoassays. The temperature dependence of the rate of protein attachment to the latex particle was unusually small - this rate increased only by 27% when the temperature was raised from 25°C to 35°C. This suggests that non-covalent protein adsorption on the polymer is rate determining. On the other hand. the rate of chloride release increases in this temperature interval by a factor of 17 and while the protein is bound to the latex particle by only 2 bonds at 25°C, 22 bonds are formed at 35°C. 

Szleifer I (1997) Protein adsorption on surfaces with grafted polymers a theoretical approach. Biophys J 72 595-612 Tanford C (1973) The hydrophobic effect. John Wiley Sons, Inc., Hoboken Van Dulm P, Norde W, Lyklema J (1981) Ion participation in protein adsorption at solid surfaces. J Colloid Interf Sci 82 77-82 Zoungrana T, Findenegg GH, Norde W (1997) Structure, stability and activity of adsorbed ensymes. J Colloid Interf Sci 190 437-448 Zoungrana T, Norde W (1997) Thermal stability and enzymatic activity of a-chymotrypsin adsorbed on polystyrene surfaces. Colloid Surf B 9 157-167... 

T. Ives and W. M. Reichert, Protein adsorption on the surface of a thin-film polymer integrated optical waveguide, Appl. Spectrosc. 42, 68-72 (1988). 

Peppas et al. [16] presented a new method for calculating protein adsorption on polymeric surfaces. In their model, protein adsorption is regarded as an equilibrium reaction, which takes place on the polymer surface in competition with the adsorption of water. 

Interestingly, protein adsorption is also a field of biological interfacial chemistry which parallels that of synthetic materials at the solid - liquid interface. A number of spectroscopic advances have been made which allow FT-IR to be used in kinetic monitoring of protein adsorption on metals and "biocompatible" polymers. In addition to providing in - situ measurements of total adsorbed protein, FT-IR can also yield information about perturbation of protein secondary structure in adsorbed layers. 

In 1982, an azo-dye-containing copolymer of 2-hydroxymethyl methacrylate was used for modulation of lysozyme adsorption on polymer beads. The amount of the protein adsorbed on a microsphere decreased upon irradiation, but rather long... 

There are several recent examples of the switching of nonspecific protein binding on polymer surfaces by application of an external stimulus. Alexander and coworkers demonstrated that protein adhesion can be controlled on PNIPAM surface brushes [14, 181]. For instance, it was reported that the adsorption of FITC-labeled bovine serum albumin (FITC-BSA) on PNIPAM/hexadecanethiol micropatterned surfaces could be tuned by LCST. However, this effect was found to be less pronounced after prolonged incubation times or repeated heating/cooling cycles. The authors suggested that this behavior could be due to unspecific PNIPAM-protein interactions [14],... 

Despite of hybridization with biological ligands, the general strategy for optimizing protein adsorption on biomaterial surfaces relies on chemical or physicochemical modulation of surface hydrophilicity [38,39]. The common approach is surface immobilization of hydrophilic polymers like polyethylene glycol [PEG] or polysaccharides. Four categories of surface-modification pathways have been developed ... 

The relative extent of protein adsorption onto polymer surfaces is influenced by the surface tensions of the substrate material, of the suspending liquid and of the proteins themselves. For one and the same substrate material the extent of protein adsorption depends on the relative hydrophobic ty of the proteins. For the situation where YlV > YPV more hydrophobic proteins will adsorb to the largest extent. 

One possible objection to the comparison of protein adsorption on flat surfaces with platelet adhesion and activation in polymer-coated bead columns is raised by the work of Vroman et al. (28) who showed that protein adsorption onto surfaces from plasma in narrow... 

Despite the criticisms above, the vOCG approach has been frequently and successfully used over recent years to interpret polymer solubility in water [14] (this is not possible using the y approach ), protein adsorption on clays [57] and conducting polymers (see Section IV.A.2 below), cell adhesion to copolymer surfaces [65], yeast-yeast and yeast-bacteria adhesion [72], fiber-matrix adhesion [69], and the hydrodynamic detachment of colloidal particles from glass plates [70]. 

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