Protein purification technolog applications

For every protein purification problem there is always an affinity solution, but cost and safety considerations may render these solutions impractical. As an example, antibodies are widely used for analysis, where only relatively small amounts are usually required, but their production and purification on a large scale for preparative-scale chromatography may be difficult to justify economically. In some cases, Hhybridoma technology may be able to address this problem. Even if production costs are acceptable, the immobilized antibodies may be unstable over the sequence of sample application, elution, and sanitation required for multiple use of the affinity adsorbent. For these reasons, while biological ligands (antibodies, enzymes, receptors, lectin. 

Nanosized particles and porous materials play an important role in many technological applications. They have been used widely as biological sensors, integrated electronic devices, catalyst supports, and adsorbents for detoxification of industrial effluents and domestic water, decoloring of processing water, and purification of pharmaceuticals and proteins. In Chapter 3, Xu et al. showed some typical applications of functionalized superparamagnetic particles in biological cell separation and industrial effluent detoxification. Porous media, on the other hand, constitute an... 

Affinity chromatography is a specific purification technology based on biological function or individual chemical structure. The application of this technique is in separation of active biomolecules from denaturated or functionally different forms in the isolation of pure proteins present at low concentration and also for removing specific contaminants [53]. 

The advent of recombinant DNA technology led to the development of antibodies and fragments that are tailored for optimal behaviour in vivo [7,8]. Humanized and chimeric antibodies can be constructed to circumvent the human anti-mouse antibody response elicited by mouse antibody treatment of patients, which severely hampers the application of these powerful molecules. The treatment of rheumatoid arthritis patients with doses of as high as 10 mg kg cA2 chimeric antibody specific for TNFa [9], emphasizes that at present the production and purification methods for these proteins have been optimized to such extent that clinical studies can be considerably intensified. 

G Zhao, TI Meier, WK Yeh. Penicillin-binding proteins as antimicrobial targets expression, purification, and assay technologies. In HA Kirst, WK Yeh, MJZmijew-ski, Jr., eds. Enzyme Technologies for Pharmaceutical and Biotechnological Applications. New York Marcel Dekker, 2001, pp. 263-287. 

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