Protein oxidation oxidative stress marker

Oxidative Protein Modification as an Oxidative Stress Marker. 222... 

A similar conclusion is drawn in a study developed on oxidative stress markers in 32 healthy subjects. Daily consumption of grape juice (10 mL/kg body weight) for 2 weeks resulted in increased resistance of LDL to ex vivo oxidation, comparable to the value obtained after a-tocopherol. Furthermore, decreased protein-carbOTiyl craicen-tration was observed at the same time [107]. These results agree with those COTitrib-uted by other authors, who showed that the daily intake of grape juice (125 mL) for... 

The antioxidant scavenger properties of brassica extracts rich in phenolic compounds have been proved in vivo in different organism. Broccoli extracts reduced the oxidation of muscle protein and liver lipids when supply to chicken in a diet (Young et al. 2002) and reduced several oxidative stress markers in healthy hiunan beings (Murashima et al. 2004). Red cabbage extracts protects against oxidative stress provoked... 

Several markers of oxidative stress have been identified in AMD retinas, including proteins modified by products of lipid peroxidation (Crabb et al., 2002 Gu et al., 2003 Hollyfield et al., 2003). Overall, there is growing body of evidence implicating oxidative stress in the development and progression of AMD (Anderson et al., 2002 Beatty et al., 2000 Seddon et al., 2004). 

Numerous in vitro studies have implicated ROS in neuronal death [Leonardi and Mytilineou, 1998], and different markers of oxidative stress are found in postmortem examination of brain tissues from patients with neurodegenerative disorders [Sayre et al., 2001], DNA oxidation, protein oxidation, and lipid peroxidation have been reported in brain regions containing neurofibrillary tangles and senile plaques from Alzheimer s disease (AD) patients [Lovell and Markesbery, 2007 Polidori et al., 2007], Dopaminergic neurons in the substantia nigra of brains of Parkinson s disease (PD) patients also exhibit hallmarks of oxidative stress [Giasson et al., 2002]. 

Oxidation of histidine yields 2-oxohistidine and its isomer 2-imidazolone, among other products (U4, U5) (Fig. 6). 2-Oxo-histidine was proposed to be a useful biological marker for assessing protein modifications under oxidative stress (U5). 

U2. Uchida, K., Kanematsu, M., Sakai, K., Matsuda, T., Hattori, N., etal., Protein-bound acrolein Potential markers for oxidative stress. Proc. Natl. Acad. Sci. USA 95, 4882-4887 (1998). 

Another marker for protein oxidation is nitration of tyrosine residues, and numerous previous studies support the notion that nitrosative stress also contributes to neurodegeneration in AD (Smith et al., 1997 Tohgi et al., 1999 Castegna et al, 2003 Sultana et al., 2006b). A number of mechanisms for tyrosine nitration of protein have been proposed, and the two widely believed to exist in vivo involve formation of peroxynitrite or mediation via hemeperoxidases (Brennan et al., 2002). These mechanisms involve NO or its by-products that react with ROS (Beckman et al. 

Mason RP, Barrett JC. Biomarkers of oxidative stress study 11 are oxidation products of Upids, proteins, and DNA markers of CC14 poisoning Free Radic. Biol. Medi. 2005 38 698. 19. 

Kadiiska MB, Gladen BC, Baird DD, et al. Biomarker of oxidative stress study II. Are oxidation products of lipids, proteins and DNA markers of CCL4 poisoning. Free Radical Biol Med. 2005 38 698-710. 

Decreased accumulation of protein carbonyls (markers of oxidative stress) in brain C57Bty6 mice... 

---