Protein Hansenula polymorpha

Steinborn, G., Boer, E., Scholz, A. et al. (2006) Application of a wide-range yeast vector (CoMed) system to recombinant protein production in dimorphic Arxula adeninivorans, methylotrophic Hansenula polymorpha and other yeasts. Microbial Cell Factories, 5, 33. 

Yeasts Easy to grow in large-scale volumes Able to glycosylate. Improved glycosylating yeast Pichiapastorisand Hansenula polymorpha Easy to achieve secretion of recombinant proteins into growth medium Gene expression can be difficult to control Some post-translational modifications may not be correct... 

Diamine oxidase occurs, like amine oxidase, in bacteria, animals, and plants [133]. The comparison of amino acid sequences of the amiloride-binding protein from human kidney, rat colon, diamine oxidase from human placenta, pig kidney, and amine oxidase from Hansenula polymorpha and lentil seeds has shown that the amiloride-binding protein and diamine oxidase are identical proteins[29]. The amiloride-binding protein was previously postulated to function as an epithelial sodium-transporter. While its physiological function is still... 

Kim S Y, Sohn J H, Pyun Y R, et al. (2002). A cell surface display system using novel GPI-anchored proteins in Hansenula polymorpha. Yeast. 19 1153-1163. 

Systematic investigations of microbial cell recovery by foam flotation were performed by Hansenula polymorpha [113-117] and Saccharomyces cerevisiae [ 118 -123] in continuous operation. The equipment used for flotation was often identical to that used for protein flotation. The microorganisms were cultivated in laboratory reactors on synthetic media in the absence of antifoam agents in continuous operation and the cell-containing cultivation medium was collected in a buffer storage and was fed into the middle of the colunm, at the top of the interface between the bubble and the foam layers. The height of the interface was controlled by an overflow. The foam left the colunm at the top. The cells were recovered from the foam liquid by a mechanical foam destroyer. The liquid residue left the column through an overflow [113] (Fig. 6). 

PI. polymorpha [80] is generally referred to as safe and was widely used for pharma-protein production (cytokines, vaccines, coagulation factors) [80]. The genome sequence is known and DNA chip technologies have been established. Hansenula was developed to an expression platform which was implemented for the production of recombinant vaccines. 

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