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Protein concentration, rate

Fig. 5. Anion-exchange separation of insulin and insulin A- and B-chains, over diethylaminoethyl (DEAF) in a 10.9 x 200 mm column having a volume of 18.7 mL. Sample volume is 0.5 mL and protein concentration ia 16.7 mAf Tris buffer at pH 7.3 is 1 mg/mL for each component ia the presence of EDTA. Eluent (also 16.7 mAf Tris buffer, pH 7.3) flow rate is 1.27 ml,/min, and protein detection is by uv absorbance at 280 nm. The straight line depicts the salt... Fig. 5. Anion-exchange separation of insulin and insulin A- and B-chains, over diethylaminoethyl (DEAF) in a 10.9 x 200 mm column having a volume of 18.7 mL. Sample volume is 0.5 mL and protein concentration ia 16.7 mAf Tris buffer at pH 7.3 is 1 mg/mL for each component ia the presence of EDTA. Eluent (also 16.7 mAf Tris buffer, pH 7.3) flow rate is 1.27 ml,/min, and protein detection is by uv absorbance at 280 nm. The straight line depicts the salt...
Fig. 32. Maximum flux obtained with various protein solutions as a function of protein concentration according to equation 3. Feed flow rates, cm /min = A, 3000 B, 2000 C, 1000 and D, 500. The flux decreases exponentially as the protein concentration increases. The extrapolated protein concentration at no flux is the gel point for this type of solution (approx 28%). These results were obtained in a flow-through cell and demonstrate the... Fig. 32. Maximum flux obtained with various protein solutions as a function of protein concentration according to equation 3. Feed flow rates, cm /min = A, 3000 B, 2000 C, 1000 and D, 500. The flux decreases exponentially as the protein concentration increases. The extrapolated protein concentration at no flux is the gel point for this type of solution (approx 28%). These results were obtained in a flow-through cell and demonstrate the...
Air flow rate, ml/min DO, mg/1 Optical density, absorbance, A520nm Cell dry weight, mg/ml Protein concentration, mg/1 Sugar concentration, g/1... [Pg.18]

Fig. 17. Cyclic voltammogram of the water-soluble Rieske fragment from the bci complex of Paracoccus denitrificans (ISFpd) at the nitric acid modified glassy carbon electrode. Protein concentration, 1 mg/ml in 50 mM NaCl, 10 mM MOPS, 5 mM EPPS, pH 7.3 T, 25°C scan rate, 10 mV/s. The cathodic (reducing branch, 7 < 0) and anodic (oxidizing branch, 7 > 0) peak potentisds Emd the resulting midpoint potential are indicated. SHE, standEU d hydrogen electrode. Fig. 17. Cyclic voltammogram of the water-soluble Rieske fragment from the bci complex of Paracoccus denitrificans (ISFpd) at the nitric acid modified glassy carbon electrode. Protein concentration, 1 mg/ml in 50 mM NaCl, 10 mM MOPS, 5 mM EPPS, pH 7.3 T, 25°C scan rate, 10 mV/s. The cathodic (reducing branch, 7 < 0) and anodic (oxidizing branch, 7 > 0) peak potentisds Emd the resulting midpoint potential are indicated. SHE, standEU d hydrogen electrode.
The intraocular pressure depends primarily on the rate of secretion of aqueous humour. The most notable con-stititutional difference between aqueous humour and blood plasma lies in their protein contents. Protein concentration in the aqueous is 5-15 mg/100 ml, and that of the plasma is 1000-fold greater (6-7 g/100 ml). However, all the plasma proteins are present in aqueous humour - albeit at very low concentrations, therefore it is... [Pg.129]

For instance, the time course of SPE demonstrates that the solvent phase surfactant concentration steadily decreases (Fig. 3) [58]. The w/o-ME solution s water content decreases at the same rate as the surfactant [58]. The protein concentration at first increases, presumably due to the occurrence of Steps 2 and 3 above, but then decreases due to the adsorption of filled w/o-MEs by the solid phase (Fig. 3) [58]. Additional evidence supporting the mechanism given above is the occurrence of a single Langmuir-type isotherm describing surfactant adsorption in the solid phase for several SPE experiments employing a given protein type (Fig. 4) [58]. Here, solid-phase protein molecules can be considered as surfactant adsorption sites. Similar adsorption isotherms occurred also for water adsorption [58]. [Pg.477]

Nasmyth Cln3 is a transcriptional activator of the Clnl and Cln2 cyclins, which are somewhat similar to cyclin E. It is a very unstable protein whose rate of synthesis is proportional to cell size. Its concentration is going to be directly and immediately proportional to the rate of synthesis. This stuff goes into the nucleus, which is roughly constant in size. [Pg.97]

In general, microsomes/S9 and cofactor (NADPH or UDPG A) are the most costly components of an incubation, but substrate, especially for early discovery compounds, is sometimes scarce. Higher enzyme concentration will lead to higher volume productivity and less amount of the cofactor to maintain the same cofactor concentration. However, the relationship of reaction rate and enzyme concentration may not be linear, so a higher enzyme concentration may yield lower enzyme productivity (amount of product per milligram enzyme used). Therefore, different protein concentration levels should be screened to obtain a good balance between volume and enzyme productivities. [Pg.204]

Ideally, milk protein concentration should be increased by enabling an increase in milk protein synthesis without decreasing milk yield or without increasing it at a rate that allows any extra protein production to be diluted. [Pg.100]

When attempting to use NMR to measure a dissociation constant, the basic experiment will be to vary the ligand concentration in the presence of a fixed concentration of protein. (The converse experiment, varying the protein concentration, may sometimes be carried out, but is generally less satisfactory because of problems with protein solubility and aggregation.) What one sees in this experiment will depend critically on the rate of... [Pg.310]

The two forms, with protein from Themiste zostericola, undergo spontaneous spectral changes (28, 31) and complete loss of EPR signal (30) by a first-order rate process, with a rate constant independent of protein concentration. This change is ascribed to a remarkable intramolecular disproportionation process within the octamer ... [Pg.222]

Statistical Methods. Means of treatment groups for plasma retention of BSP, plasma osmolality, total plasma protein concentration and urine flow rates were compared by students t test for independent sample means (17). Plasma enzyme activity data were converted to a quantal form and analyzed by the Fischer Exact Probability Test (18). Values greater than 2 standard deviations (P < 0.05) from the control value were chosen to indicate a positive response in treated fish. [Pg.403]


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See also in sourсe #XX -- [ Pg.70 ]




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