Protein-coding sequence

E3. Eto, K., Sakura, H., Yasuda, K., Hayakawa, T Kawasaki, E Moriuchi, R., Nakataki, S., Yaza-ki, Y., and Kadowaki, T Cloning of a complete protein-coding sequence of human platelet-type phosphofructokinase isozyme from pancreatic islet. Biochem. Biophys. Res. Commun. 198, 990-998 (1994). 

SNPs, or single nucleotide polymorphims, are the subtle changes in the genome sequence that can lead to alterations either within single proteins by altering the protein coding sequence, or whole pathways, if the mutation affects... 

To make mRNA, the primary transcript must be spliced to bring the protein-coding sequences (exons) together and to remove the intervening sequences (introns). The splice signals consist of a 5 and a 3 set of sequences that are always found at splice junctions. However, this is generally believed to provide too little information to recognize a splice site specifically and correctly. Some sequences in the intron are also important. 

Various verification steps have been introduced to ensure that SPTR is comprehensive and contains all relevant data sources. The main source of new protein sequences is the translations of CDS in the nucleotide sequence databases. The up-to-date inclusion of new protein sequence entries is ensured by the weekly translation of EMBL-NEW (the updates to the EMBL nucleotide sequence database). The three collaborating nucleotide sequence databases DDBJ, EMBL, and GenBank exchange their data on a daily basis. Therefore any protein coding sequence submitted to DDBJ/EMBL/GenBank will appear in SPTR within 2 weeks in the worst case and within less than 1 week in the average case. 

INDIVIDUAL VARIATION IN PROTEIN-CODING SEQUENCES OF HUMAN GENOME... 

Codon A set of three nucleotides in a protein coding sequence that specifies individual amino acids or a termination signal (codon, terminator). Most codons are universal, but some organisms do not produce the transfer RNAs (RNA, transfer) complementary to all codons. These codons are referred to as unassigned codons (codons, nonsense). [NIH]... 

Cleave multi-drug protein coding sequence... 

Blastocystis hominis is a unicellular anaerobic organism that inhabits the human intestinal tract, and whose metabolism and life cycle are not completely understood. Molecular phylogenetic analysis of the SSU rRNA sequences (Silberman et al. 1996) and protein-coding sequences (Arisue et al. 2002c) show Blastocystis branching within stramenopiles (heterokonts), a group containing a vast diversity of photosynthetic and heterotrophic protists (Fig. 10.2). 

Rogozin, I. B D Angelo, D Milanesi, L. (1999). Protein-coding regions prediction combining similarity searches and conservative evolutionary properties of protein-coding sequences. Gene 226, 129-37. 

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