Intervening sequence

Exons are protein-coding regions that mu.st be joined by removing introns, die noncoding intervening. sequences. 

Rowland, L.J. Strommer, J.N. (1985). Insertion of an unstable element in an intervening sequence of maize AdhI affects transcription but not processing. Proceedings of the National Academy of Sciences, USA, 82, 2875-9. 

Coding Regions Are Often Interrupted by Intervening Sequences... 

The protein coding regions of DNA, the transcripts of which ultimately appear in the cytoplasm as single mRNA molecules, are usually interrupted in the eukaryotic genome by large intervening sequences of... 

Oxidant/ Reductant Evidence for CT Injection Site/ Intervening Sequence krt/Distance Regime Distance Dependence Sequence Specificity... 

Ru(dppz)(x) (y)3+/ Me, tryptophan AG -0.6 V detect G(-H), M, and tryptophan radical s by transient absorption, EPR Gs in intervening sequence k 107 s 1 up to 50 A CT not rate limiting up to 50 A GGox varies with sequence, base-stack pertubrations and extent of intercalation... 

Et /ZG AG -0.2 V fluorescence quenching correlates with CT driving force no spectral overlap for energy transfer no Zs in injection site or intervening sequence k = 2 x 1011 s 1 observed up to 20 A in time-resolved and steady-state experiments shallow dependence of CT yield between 6-24 A (y = 0.2-0.3) no dependence of kci between 10-17 A strong efficiency and distance dependence of CT yield vary with Z flanking sequence and intervening mismatches... 

St /G AG-0.14 V fluorescence quenching stilbene radical anion detected in transient absorption no Gs near injection site or in intervening sequence k = 1012-108 s 1 for 0-4 intervening A-T base-pairs (-3.4-17 A) exponential distance dependence of CT rate constant (3 0.6-0.7 A"1 small variations in k depending on whether G is in the A or T arm of the hairpin... 

With the site-selective hole injection and the hole trapping device established, the efficiency of the hole transport between the hole donor and acceptor, especially with respect to the distance and sequence dependence, were examined. Our experiments showed that hole transport between two guanines was extremely inefficient when the intervening sequence consisted of more than 5 A-T base pairs [1]. Hole injection into the DNA n-stack using photoexcited dCNBPU was accompanied by the formation of dCNBPU anion radical. Therefore, hole transport would always compete with the back electron transfer (BET). To minimize the effect of BET, we opted for hole transport between G triplets, that are still lower in oxidation potential than G doublet. With this experimental system, we researched the effect of the bridging sequence between two G triplets on the efficiency of hole transport [2]. 

In a separate experiment, we confirmed the cleavage at 5 G of the GG step in opposite strand ODN 12. The presence of G in the intervening sequence... 

To make mRNA, the primary transcript must be spliced to bring the protein-coding sequences (exons) together and to remove the intervening sequences (introns). The splice signals consist of a 5 and a 3 set of sequences that are always found at splice junctions. However, this is generally believed to provide too little information to recognize a splice site specifically and correctly. Some sequences in the intron are also important. 

Brush, D., Dodgson, J.B., Choi, O.R., Stevens, P.W., and Engel, J.D. (1985) Replacement variant histone genes contain intervening sequences. Mol. Cell. Biol. 5, 1307-1317. 

Wells, D. and Kedes, L. (1985) Structure of a human histone cDNA Evidence that basally expressed histone genes have intervening sequences and encode polyadenylylated mRNAs. Proc. Natl. Acad. Sci. USA 82, 2834-2838. 

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