Proliferation Cell count

In leukemia the CD34+117+38- primitive progenitor cell counts were elevated mainly in patients with proliferation of poorly differentiated cells while in ALL s the CD34+ counts were smaller (M=0,820, SD=0,185). 

Griffey, S. M., Kraegel, S. A., and Madewell, B. R., 1999. Proliferation indices in spontaneous canine lung cancer proliferating cell nuclear antigen (PCNA), Ki-67 (M1B1) and mitotic counts. 7. Comp. Pathol. 720 321-332. 

Keshgegian, A.A. and Cnaan, A. 1995. Proliferation markers in breast carcinoma. Mitotic figure count, S-phase fraction, proliferating cell nuclear antigen, Ki-67 and MIB-1. Am. J. Clin. Pathol. 104, 42-49. 

Surprisingly, no difference in immune function as measured by spleen cell counts, in vitro lymphocyte proliferation responses to mitogens, and in vitro and in vivo plaque-forming cell responses to antigens was observed (30). However, human studies have shown changes in natural killer cell activity after TSD and during RS (31,32). 

The potential association between the immune system and mood disorders has become a major topic of interest in biological psychiatry in the past decade. In general, three immune measures have been examined, namely white blood cell counts, functional measures of cellular immunity such as natural killer cell activity and immune cell markers as exemplified by human lymphoctye antigen (HLA). The cumulative data from these studies suggests that depressed patients have a decreased number of lymphocytes, reduced mitogen-induced lymphocyte proliferation and a reduction in the number of natural killer cells. However, this does not apply to all depressed patients. Furthermore, not all aspects of immune function... 

Cell concentration in suspension can be determined through an optical microscope employing a hemocytometer for manual cell counting, or in a semi-automatic way using an electronic particle counter (such as a Coulter counter), as described in detail by Freshney (2005). Through dye exclusion (such as trypan blue), it is possible to determine viable cell concentration, that is the number of cells in a known sample volume capable of proliferating in favorable culture conditions. 

The critical importance the CD4+ T cells and their messenger molecules for B-cell proliferation is manifest in AIDS where HIV proteins promote death of CD4+ T cells resulting in inadequate stimulation of B-cell proliferation. One of the early clues to this action of HIV was the change in the ratio of CD4 to CD8 cells in AIDS patients. The CD4+ cell count and the CD4/CD8 ratio quickly became markers for the progression of the disease. The immunodeficiency that results from destruction of Th cells... 

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