Preparation of 2 Stock Solution

STEP 1 PREPARATION OF STOCK SOLUTIONS. The aqucous Cd solution is prepared as for CdS. As for CdS, the nature of the anion does not appear to be very important—the sulphate is commonly used. 

Data regarding preparation of stock solutions available ... 

Chemicals, Preparation of Stock Solutions and Working Solutions... 

Stock solutions of the drug and the internal standard were prepared in methanol in a concentration of 2 mM (docetaxel) and 1 mM in case of the internal standard, paclitaxel. A separate stock solution was made up for the preparation of the calibration standards and quality control samples in order to uncover possible errors during the preparation of stock solutions. 

Preparation of Stock Solutions and Working Solutions Separate stock solutions of the drug, all three metabolites and the internal standard were prepared in water/methanol (70 30, v/v) to a concentration of 8 mg/mL in case of CP and D4-CP and 0.5 mg/mL in case of the three metabolites. 

Two separate stock solutions were made up for the preparation of the calibration standards and quality control samples, in order to uncover possible errors during the preparation of stock solutions. 

The only manual operations required for the synthesis of this library were the preparation of stock solutions of 8.25a,b (0.5 M THF) and the monomer representatives Ml (1.5MDlPEAand 3MTHF) and M2 (1 MTHF). The other operations... 

The chemical reagents used for the preparation of stock solutions were reagent grade and were used without further purifieation. The chemicals were in the form of the nitrate salts and were obtained eommercially from Aldrich or Fluka. Pure ceria samples were prepared by precipitation of ceria from aqueous solutions containing O.OIM Ce by adding 1 M NH3 solution. The precipitate was dried at 523K. The surface acid sites concentration was measured using Hammett titrations, with phenolphthalein as indicator, and a O.IM NaOH solution as base. 

The chemical reagents used for the preparation of stock solutions were reagent grade and were used without further purification. The chemicals were in the form of the nitrate salts and were obtained commercially from Aldrich or Fluka. 

For the preparation of stock solutions for Fehling s reagent, see Appendix. 

Ecdysteroids All ecdysteroids used in this work were a kind gift of Prof. Dr. Jan Koolmann, University of Marburg. They were ecdysone, 20-hydroxyecdysone, makisterone and muristerone for the growth experiments, as well as radiolabeled ponasterone A for the binding assay. Preparation of stock solutions, sterilization and addition to media was exactly the same as for brassinosteroids. 

DMSO (for preparation of stock solutions of cannabinoid ligands). 

Preparation of Stock Solutions of Amphiphiles. Stock solutions for SAM formation were prepared by dissolving the ODP in a 100 0.4 (vA ) mixture of 7i-heptane and propan-2-ol at concentrations of 5, 50, and 500 fjM, followed by sonication for 10 min. For comparison purposes, a 500 fjM solution was also prepared in pure propan-2-ol (ODP was found to be insoluble in pure /i-heptane). The resulting solutions were filtered through a 200 nm cellulose nitrate membrane and stored at ambient temperature in glass bottles until use. No deleterious effects were observed upon storage of these solutions for up to several weeks. 

TABLE 1 Preparation of stock solution and analytical methods used for study chemicals. 

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