Porcine plasma protein

Benjakul, S., Visessanguan, W., and Srivilai, C., Porcine plasma protein as proteinase inhibitor in big eye snapper (Priacanthus tay emus ) muscle and surirni, J. Sci. Food Agric., 81, 1039, 2001b. 

Lertittikul, W., Benjakul, S., and Tanaka, M. Characteristics and antioxidative activity of Maillard reaction products from a porcine plasma protein-glucose model system as influenced by pH, Food Chem., 100, 669, 2007. 

Nuthong, P. Benjakul, S. Prodpran, T. Effect of phenolic compounds on the properties of porcine plasma protein-based film. Food Hydrocolloids 2009, 23, 736-741. 

For sample preparation, the I.S. (d7-TACA) was spiked into each 1 mL portion of porcine plasma sample to a concentration of 100 pg/mL then mixed 1 1 (v v) with 4 % phosphoric acid to release protein-bound drugs. SPE cartridges were preconditioned as described above, after sample loading, optimized SPE wash and elution methods were applied for sample extraction. 

Sample Preparation A 200 pL sample of porcine plasma was spiked with 2pg/mL drug mixture and then precipitated with 800 pL acetonitrile and filtered through a Phenomenex Impact Protein Precipitation Plate Onyx Monolithic C18 100 x 4.6 mm CHO-7643... 

The sequences shown are those of porcine and human angiotensins in the bovine peptide position 5 is occupied by valine rather than isoleucine. Treatment of the intact plasma protein, which is the source of the angiotensins, with trypsin yields a 14-peptide, angiotensinogen, itself a good substrate for renin [2] ... 

Our proposal is not theoretical. Researchers have used reticulated hydrophobic polyurethanes as liver assist devices with some success. We will discuss this research and future work in detail later. For now, it is useful to present an overview. Matsushita et al. inoculated a reticulated polyurethane with porcine hepatic cells, "fhe device functioned as noted, but it was necessary to separate the plasma from the blood because conventional hydrophobic polyurethanes are not hemocompatible. In addition, the technique made no provision for cell attachment. Workers in our laboratory grafted a hydrophilic polyurethane to the structural members of a hydrophobic reticulated foam in an effort to make the composite hemocompatible. Additionally, this gave us the opportunity to add cell attachment proteins. 

OH)2D3-mediated phosphorylation [67]. In the case of PKC, 1,25-(OH)2D3 causes translocation of PKC-/ to the plasma membrane and PKC-a to the nucleus [269-271], Both PKC iso types are calcium-dependent, and l,25-(OH)2D3 does not affect the calcium-independent PKC-f [271], Brown and DeLuca [272] demonstrated that VDR phosphorylation is rapid, occurring within 1 h following treatment of l,25-(OH)2D3 of embryonic chick duodenal organ culture. In addition, phosphorylation occurred before calcium uptake and the 1,25-(OH)2D3-dependent increase in calcium-binding protein mRNA. Proteolytic digestion of the l,25-(OH)2D3-dependent phosphorylation of porcine VDR was mapped and shown to be localized to a 23 Kd fragment of the C-terminal domain [273]. 

Recently, the cofactor peptides have also been isolated from semicarbazide-sensitive amine oxidases purified from bovine and porcine aortas [52], sequenced and confirmed to contain the topa quinone. The same topa quinone consensus sequence was also found in the primary structures of amine oxidases from human kidney [53], human retina [54] and rat colon [55], so called amiloride-binding proteins , and amine oxidase from human placenta [56] that shows 81% identity with bovine plasma amine oxidase [57], bovine lung amine oxidase [58], and amine oxidases from pea and lentil seedlings [59,60], chick pea seedlings [61], and Arabidopsis thaliana [62] obtained by the molecular cloning of respective DNAs. 

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