Polysaccharide-degrading

Zollfrank (2001) reacted beech wood with trimethylsilyl chloride under anhydrous conditions in tetrahydrofuran in the presence of pyridine. Pyridine was present to scavenge the HCl produced during the reaction, thus limiting polysaccharide degradation. After reaction at room temperature for 12 hours, the wood was extracted with methanol to remove the pyridinium chloride by-product. SEM of the modified wood indicated that there was no evidence of cell wall degradation. 

Liquid-chromatographic techniques have been useful for studying the mode of action of various polysaccharide-degrading enzymes. Rapid and quantitative, l.c. end-product analysis has been used to study the action... 

Polysaccharide degrading enzymes have a long history of commercial application in food processing, horticulture, agriculture, and protein research. As with most other industrial enzymes, the economic use of polysaccharidases often depends on obtaining the maximum activity lifetime in the process environment and/or securing a recovery system that permits the sensible reuse of active enzymes from process streams. 

Jarvis, M. C., Threlfall, D. R., Friend, J. (1981b). Potato cell wall polysaccharides degradation with enzymes from Phytophthora infestans. J. Exp. Bol, 32,1309-1319. 

Acids act similarly and rapidly to permanently disrupt the glyeosidic bonds. Thus polysaccharides degrade to their original monosaccharide units when heated with aqueous acids. Enzymes catalyze this degradation and provide both plants and animals with a source of glucose. 

Choct, M., Hughes, R., Trimble, R.P., Angkanapom, K. and Annison, G. (1995) Non-starch polysaccharide-degrading enzymes increase the performance of broiler chickens fed wheat of low apparent metabolizable energy. Journal of Nutrition 125, 485-492. 

Magnesium compounds retard cellulose depolymerization by deactivating the transition metal catalysts. In alkaline media where hydroxy-acids or products of polysaccharide degradation are present, magnesium forms stable complexes with transition metals. The formation of iron-magnesium complexes in particular is supported by interactions approximating the coordination number of iron (6) and one half the coordination number (3). 

Kubicek, C., and Penttila, M. 1998. Regulation of production of plant polysaccharide degrading enzymes by Trichoderma. In Harman, G., and Kubicek, C. (Eds.), Trichoderma and Gliocladium, 2 (pp. 49-72). London Taylor and Francis Ltd. 

More knowledge of physical and chemical properties of pectic enzymes, their substrate specificity, pattern of action, action on plant tissues, interaction with each other and other polysaccharide degrading enzymes is essential to establish their technological roles and to improve existing applications and to develop new applications. 

Y. Aoki and Y. Kamei, Preparation of recombinant polysaccharide-degrading enzymes from the marine bacterium, Pseudomonas sp. ND137 for the production of protoplasts of Porphyra yezoensis, Eur. J. Phycol., 41 (2006) 321-328. 

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