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Polynucleotide Formation

As discussed by Pdrschke one can distinguish several basic features of helix-coil transitions in polynucleotides. Formation of a helix from separated complementary... [Pg.333]

Hawkins CL, Davies MJ (2002) Hypochlorite-Induced Damage to DNA, RNA, and Polynucleotides Formation of Chloramines and Nitrogen-Centered Radicals. Chem Res Toxicol 15 83... [Pg.490]

Besides the MDL Molfile formal, other file formats are often used in chemistry SMILES has already been mentioned in Section 2.3.3. Another one, the PDB file format, is primarily used for storing 3D structure information on biological macromolecules such as proteins and polynucleotides (Tutorial, Section 2.9.7) [52, 53). GIF (Crystallographic Information File) [54, 55] is also a 3D structure information file format with more than three incompatible file versions and is used in crystallography. GIF should not be confused with the Chiron Interchange Formal, which is also extended with. cif. In spectroscopy, JCAMP is apphed as a spectroscopic exchange file format [56]. Here, two modifications can be... [Pg.45]

PDB file. pdb Protein Data Bank file format for 3D stmcture information on proteins and polynucleotides nmm.rcsb.org 53... [Pg.46]

Section 28 14 The nucleotide sequence of DNA can be determined by a technique m which a short section of single stranded DNA is allowed to produce its complement m the presence of dideoxy analogs of ATP TTP GTP and CTP DNA formation terminates when a dideoxy analog is incorporated into the growing polynucleotide chain A mixture of polynucleotides dif fermg from one another by an incremental nucleoside is produced and analyzed by electrophoresis From the observed sequence of the comple mentary chain the sequence of the original DNA is deduced... [Pg.1189]

FIGURE 11.29 The vicinal—OH groups of RNA are susceptible to nucleophilic attack leading to hydrolysis of the phosphodiester bond and fracture of the polynucleotide chain DNA lacks a 2 -OH vicinal to its 3 -0-phosphodiester backbone. Alkaline hydrolysis of RNA results in the formation of a mixture of 2 - and 3 -nucleoside monophosphates. [Pg.346]

FIGURE 19.28 The condensation of nucleotides that leads to the formation of a nucleic acid, a polynucleotide. [Pg.896]

In a self-reproducing, catalytic hypercycle (second order, because of its double function of protein and RNA synthesis) the polynucleotides Ni contained not only the information necessary for their own autocatalytic self-replication but also that required for the synthesis of the proteins Ei. The hypercycle is closed only when the last enzyme in the cycle catalyses the formation of the first polynucleotide. Hypercycles can be described mathematically by a system of non-linear differential equations. In spite of all its scientific elegance and general acceptance (with certain limitations), the hypercycle does not seem to be relevant for the question of the origin of life, since there is no answer to the question how did the first hypercycle emerge in the first place (Lahav, 1999). [Pg.226]

So we are still left with two models of the stereochemistry of DNA alkylated by a PAH diol epoxide the PAH either lies in a groove of DNA or else tries to intercalate between the bass of DNA. Since it is covalently bonded to a base it must cause considerable distortion if it tries to lie between the bases. However, the stacking observed in the crystalline state seems to argue for partial intercalation. We will need crystal structures of at least one appropriately alkylated polynucleotide before this problem can be resolved. And when this is done it will be just the beginning of the answer to the problem of alkylation of DNA by activated carcinogens. The subsequent question is, what is the lesion in DNA that is important in carcinogenesis, and then what does it cause to happen so that tumor formation is initiated ... [Pg.181]

By hydrolysis under very mild alkaline conditions (with a boiling suspension of barium carbonate), ribonucleic acids have been shown to yield small quantities of cyclic phosphates as well as the normal nucleotides.96 These materials were identical electrophoretically with synthetic cyclic phosphates and were readily hydrolyzed to mixtures of 2- and 3-phosphates. Their formation in this way constitutes strong support for Brown and Todd s theory. The precise way in which the alkaline hydrolysis of the polynucleotide occurs has been studied using isotopically labeled water, and the results are in agreement202 with the scheme outlined above. [Pg.322]

Sequence analysis of the 5 -ends of viral and nuclear mRNA molecules reveals that these are frequently capped , with an unusual structure in which 7-methylguanosine is joined by a (5 - 5 ) triphosphate link to a 2 -0-methyl nucleoside moiety which is the first residue in the (3 ->5 )-linked polynucleotide chain.166-168 The presence of this cap structure is required for ribosomal binding and translation to take place,167- 168 and 7-methylguanosine-5 -phosphate inhibits translation by preventing formation of the ribosome-mRNA complex.169... [Pg.174]

Maurer N, Wong KF, Stark H, et al. Spontaneous entrapment of polynucleotides upon electrostatic interaction with ethanol destabilized cationic liposomes formation of small multilamellar liposomes. Biophys J 2001 80 2310. [Pg.146]

The formation of the transient reduced complex can also be observed by flash photolysis in the presence of CT-DNA [1(X)]. This shows clearly the existence of a photoinduced electron transfer from a base of the polynucleotide to the excited complex. However, the relative amount of reduced complex which is photoproduced, is smaller in the presence of CT-DNA than in the presence of GMP this may be attributed to a more important back electron transfer process in the ion pair produced on the polynucleotide compared to that in solution with the mononucleotide. [Pg.53]

In addition to the enzymes that catalyse the formation of nucleotides and polynucleotides, a large number of catabolic systems exist which operate at all levels of the internucleotide pathways. The ribonucleases and deoxyribonucleases that degrade polynucleotides are probably not significantly involved in purine analogue metabolism, but the enzymes which dephosphorylate nucleoside 5 -monophosphates are known to attack analogue nucleotides and may be of some importance to their in vivo activity. Phosphatases of low specificity are abundant in many tissues [38], particularly the intestine [29]. Purified mammalian 5-nucleotidases hydrolyse only the nucleoside 5 monophosphates [28] and... [Pg.85]

These three compounds exert many similar effects in nucleotide metabolism of chicks and rats [167]. They cause an increase of the liver RNA content and of the nucleotide content of the acid-soluble fraction in chicks [168], as well as an increase in rate of turnover of these polynucleotide structures [169,170]. Further experiments in chicks indicate that orotic acid, vitamin B12 and methionine exert a certain action on the activity of liver deoxyribonuclease, but have no effect on ribonuclease. Their effect is believed to be on the biosynthetic process rather than on catabolism [171]. Both orotic acid and vitamin Bu increase the levels of dihydrofolate reductase (EC 1.5.1.4), formyltetrahydrofolate synthetase and serine hydroxymethyl transferase in the chicken liver when added in diet. It is believed that orotic acid may act directly on the enzymes involved in the synthesis and interconversion of one-carbon folic acid derivatives [172]. The protein incorporation of serine, but not of leucine or methionine, is increased in the presence of either orotic acid or vitamin B12 [173]. In addition, these two compounds also exert a similar effect on the increased formate incorporation into the RNA of liver cell fractions in chicks [174—176]. It is therefore postulated that there may be a common role of orotic acid and vitamin Bj2 at the level of the transcription process in m-RNA biosynthesis [174—176]. [Pg.290]

The reason for this phase effect is the apparent necessity for the proximity and proper orientation of two monomer molecules before dimer formation can take place (the mechanistic significance of this will be discussed later). Thus dinucleotides such as TpT form dimers even in solution, and the yield of dimers in polynucleotides and nucleic acids is even higher. [Pg.195]

The cross sections for formation of the two dimers are similar, but not identical, with those for the formation of two of the dimers from UpU, and the wavelength dependencies are similar. Hydrate formation occurs more rapidly in d-UpU than in UpU. The marked quantitative and qualitative differences in the photochemistry of these two closely similar dinucleotides, along with the additional difference in results observed with poly U, to be discussed below, make it clear that the factors influencing the course of the photolysis of polynucleotides are not well known. It must be pointed out that the structures of the various... [Pg.234]


See other pages where Polynucleotide Formation is mentioned: [Pg.32]    [Pg.32]    [Pg.1172]    [Pg.122]    [Pg.1172]    [Pg.10]    [Pg.61]    [Pg.146]    [Pg.237]    [Pg.411]    [Pg.97]    [Pg.240]    [Pg.319]    [Pg.321]    [Pg.325]    [Pg.328]    [Pg.254]    [Pg.148]    [Pg.148]    [Pg.150]    [Pg.172]    [Pg.174]    [Pg.131]    [Pg.133]    [Pg.189]    [Pg.58]    [Pg.66]    [Pg.112]    [Pg.92]    [Pg.334]    [Pg.69]    [Pg.212]    [Pg.242]   


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Polynucleotide

Polynucleotides

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