Polynucleotide-binding

Polynucleotides bind lanthanides more strongly than mononucleotides and tRNA providing stronger ligands than DNA. This difference is reflected in the data on Kd values forTb3+ complexes given in Table 11.14. 

Mellon WS, Specificity of dye-hgand interaction with the polynucleotide binding domain of 1,25-dihydroxyvitamin D3-receptor complexes of chicken intestinal cytosol, Mol. Pharmacol. 1984 25 86—91. 

J. E. Summerton, Stereoregular polynucleotide binding polymers, PCT Int. Appl. WO 86/05518, 25 September 1986 E.P. Sdrchak, J.E. Summerton, and D.D. Weller, Uncharged stereoregular nucleic acid analogues, J. Org. Chem. 52 4202 (1987). 

With respect to template-directed syntheses, the syntheses of oligonucleotide using polynucleotide have already been attempted62-69. However, it has not necessarily been successful yet, since the binding constant of the nucleotide monomer with polynucleotide was not large, particularly in aqueous media. 

The nature of the target to be attacked by any drug obviously depends on the specific application. Many cytotoxic metal complexes target DNA because of its importance in replication and cell viability. Coordination compounds offer many binding modes to polynucleotides, including outer-sphere noncovalent binding, metal coordination to nucleobase and phosphate backbone... 

Base Specificity of Physical Binding. To determine whether the physical binding of hydrocarbon metabolites to DNA exhibits base specificity, the binding of trans-7,8-dihydroxy-7,8,9,10-tetrahy-dro-BP was examined using fluorescence and absorption techniques (9). A comparison was also made of the varying degrees to which different synthetic polynucleotides are able to solubilize BPT... 

There are both geometric and kinetic differences between the interactions of A- and A-[Ru(bpy)2(hpimp)]2+ hpimp=2-(2-hydroxyphenyl)imi-dazo [4,5-/]-1,10-phenanthroline and the hexanucleotide d(GTCGAC)2. The A-isomer penetrates more deeply into the major groove of the polynucleotide it also binds more rapidly than the A-isomer to DNA (156). 

Luk KFS, Maki AH, Hoover RJ (1975) Studies of heavy metal binding with polynucleotides using optical detection of magnetic resonance. Silver binding. J Am Chem Soc 97 1241-1242... 

Nucleic acids. Immobilized polynucleotides can be used to extract nucleic acid binding proteins as well as complementary strands of nucleic acids. 

Gribskov, M. (1992). Translational initiation factors IF-1 and eIF-2 alpha share an RNA-binding motif with prokaryotic ribosomal protein SI and polynucleotide phosphoryl-ase. Gene 119, 107-111. 

Sequence analysis of the 5 -ends of viral and nuclear mRNA molecules reveals that these are frequently capped , with an unusual structure in which 7-methylguanosine is joined by a (5 - 5 ) triphosphate link to a 2 -0-methyl nucleoside moiety which is the first residue in the (3 ->5 )-linked polynucleotide chain.166-168 The presence of this cap structure is required for ribosomal binding and translation to take place,167- 168 and 7-methylguanosine-5 -phosphate inhibits translation by preventing formation of the ribosome-mRNA complex.169... 

F. Brun, J. J. Toulme, and C. Helene, Interactions of aromatic residues of proteins with nucleic acids. Fluorescence studies of the binding of oligopeptides containing tryptophan and tyrosine residues to polynucleotides, Biochemistry 14, 558-563 (1975). 

The study demonstrated that single-stranded polynucleotide, such as poly(A), is more flexible than a generic dsDNA. On mica, the long B-DNA binds to mica at many sites and remains intact during drying, while the short DNA binds at fewer sites and settles to an A-DNA structure during dehydration. 

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