Silver binding

The use of silver-based stains increases the detection sensitivity up to 100 fold, with individual bands containing as little as lng of protein usually staining well. However, because silver binds to protein non-stoichiometrically, quantitative studies using densitometry cannot be undertaken. 

Janes, N. and R.C. Playle. 1995. Modelling silver binding to gills of rainbow trout. (Oncorhynchus mykiss). Environ. Toxicol. Chem. 14 1847-1858. 

Kumar, R. and S.R. Bawa. 1979. Hepatic silver binding protein (Ag BP) from sparrow (Passer domesticus). Experientia 35 1621-1623. 

Luk KFS, Maki AH, Hoover RJ (1975) Studies of heavy metal binding with polynucleotides using optical detection of magnetic resonance. Silver binding. J Am Chem Soc 97 1241-1242... 

The following procedures are well improved and easy to perform. Nevertheless, in some cases variations of the protocols concerning silver binding and/or reduction can give better or other results, because proteins bind silver differently and are observed with varying colors. Some hints are given in the cited papers. 

AG3, AYSSGAPPMP PF, an inorganic-binding peptide silver-binding peptides) that specifically and selectively binds to silver. AG3 was immobilized on the surface of protonated poly(ethylene terephtha-late) (PET) film which was prepared for biomimetic synthesis of silver particles in vitro. Silver crystallites have been formatted on the surface of the AG3 -PET film showing various shapes 1 to 4 /rm in size [Z. Xu et al., J. Inorg. Biochem. 2005, 99,1692]. 

Ca +, Na+, H+) and complexing agents (dissolved organic carbon, thiosulfate, chloride) these variables must be considered when con-stmcting predictive models of silver binding to gills. 

The extent of absorption of an administered dose of silver depends on silver speciation, the presence and extent of silver-binding proteins, and other variables. But absorption is dependent mainly on the transit time through the gastrointestinal tract the faster the transit time, the less silver is absorbed. Transit times ranged from about 8 h in mice and rats to about 24 h in monkeys, dogs, and humans. Route of administration affected the excretion rate of silver. Clearance of silver from mammals 2 days after silver was admmistered intravenously ranged... 

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