Nucleic acid-binding proteins

Nucleic acids. Immobilized polynucleotides can be used to extract nucleic acid binding proteins as well as complementary strands of nucleic acids. 

Berg,J. M. (1986). Potential metal-binding domains in nucleic acid binding proteins. Science 232, 485-487. 

Nucleic acid. complementary base sequence, histones, nucleic acid pol3rmerase, nucleic acid binding protein. 

Heparin-Sepharose Nucleic acid-binding proteins, restriction endonucleases, lipoproteins... 

Liu, J. (2000). X-ray crystallographic studies of two nucleic-acid binding protein/ complex systems and one collagen-like peptide. Ph.D. thesis, Rutgers University. 

Blot overlays include the probing of membrane with various molecules to detect the presence of specific binding domains, for example, with guanine triphosphate (25,26) or proteoglycans (27). In the Southern or North Western blotting, the membrane is probed with deoxyribonucleic acid or ribonucleic acid molecules to detect nucleic-acid binding proteins (28). 

Guillonneau, F., Labas, V., Auvin, C., and Praseuth, D. (2001) A reliable and simple method for two-dimensional electrophoresis and identification of HeLa nuclear alkaline nucleic acid-binding proteins using immobilized pH gradient. Electrophoresis 22, 4391-4403. 

Nucleic acids Exo- and endonucleases, polymerases and other nucleic acid-binding proteins... 

Citovsky, V., Knorr, D., Schuster, G., and Zambryski, P. "The P30 movement protein of tobacco mosaic virus is a single-strand nucleic acid binding protein". Cell 60, 637-647 (1990). 

Protein-DNA Complexes. Circular dichroism has been used to characterize protein secondary structure in many kinds of nucleic acid-binding proteins, their mutants, and derivatives. Similarly, CD has been employed to characterize the structural changes which occur in the nucleic acids as well, but these are beyond the scope of the present work. Our discussion here will center on a few examples of the use of CD for characterizing structural changes which occur in DNA-binding proteins. 

As summarized in this chapter, zinc-containing nucleic acid-binding proteins are ubiquitous in nature and play integral roles in aU aspects of nucleic acid metabolism, including DNA replication, recombination and repair, transcriptional... 

On the interior of the lipid bilayer, a complex of protein and nucleic acid is found. This complex is usually referred to as a nucleocapsid core, and in many instances has an organized protein shell within which nucleic acid, possibly in complex with additional proteins, is found. These additional proteins may be nucleic acid-binding proteins as well as proteins necessary for genome replication. Some of the larger DNA viruses include many additional proteins within this core. 

The mobility shift assay (or gel retardation assay) is based on the difference in mobility between naked RNA and proteins-RNA complexes when migrating in a native polyacrylamide gel. The increased size of the complex and the usually low or positive charge of nucleic acid binding proteins give rise to the difference in mobility. 

Phosphorothioate oligonucleotides can interact with nucleic acid binding proteins such as transcription factors and single-strand nu-cldc acid binding proteins. However, very little is known about these binding events. Additionally, it has been reported that phos-phorothioates bind to an 80-kDa membrane protein that was suggested to be involved in cellular uptake processes (87). However, again, little is known about the affinities, sequences, or structure specificities of these putative interactions. More recently, interactions with 30- and 46-kDa surface proteins in T15 mouse fibroblasts were reported (170). 

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