Polynuclear aromatic hydrocarbons HPLC analysis

In LIF detection systems, excitation power may be increased up to six orders of magnitude compared to CF detection. Most LC-LIF detection concerns under-ivatised polynuclear aromatic hydrocarbons (PAHs) and fluorescing dyes (e.g. polymethines). Because only a limited number of analytes possess native fluorescence, derivatisation of the analyte before detection is normally required in trace analysis of organic solutes by means of LIF detection. LIF detection in HPLC was reviewed... 

Black, J.J., T.F. Hart, Jr., and E. Evans. 1981. HPLC studies of PAH pollution in a Michigan trout stream. Pages 343-355 in M. Cooke and A.J. Dennis (eds.). Chemical Analysis and Biological Fate Polynuclear Aromatic Hydrocarbons. Fifth International Symposium. Battelle Press, Columbus, OH. 

Analysis of Carcinogens. HPLC methods for the determination of polynuclear aromatic hydrocarbons benzidine and 3,3 -dichlorobenzidine and 4,4 -methylenebis(2-chloroaniline) (MOCA) are discussed. 

Figure 7.13. HPLC analysis of polynuclear aromatic hydrocarbons (PAHs) in a marine sediment (NIST SRM 1941) using programmed fluorescence detection. Reprinted with permission...

Polynuclear aromatic hydrocarbons at trace concentrations may be analyzed by HPLC, GC and GC/MS by US EPA Methods 610, 8100, 8270 and 8320 (US EPA 1997). GC techniques involve the use of a flame ionization detector and a fused silica capillary column or a packed column such as 3% OV-17 on Chromosorb W-AW or equivalent. Certain PAH compounds may coelute on a GC column. This problem does not occur in the reversed phase HPLC analysis using UV and fluorescence detectors. A column such as HC-ODS Sil-X or equivalent having a 5-/Lim particle size is suitable for separating PAH mixtures. GC/MS is a confirmative method to identify individual compounds although some of the compounds produce the same characteristic masses. The compounds should be identified from fheir characferisfics mass ions and refenfion times. Various analytical methods and the characteristic mass ions of some... 

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