Polymerase chain reaction strategy

Arnheim, N. Erlich, H. (1992) Polymerase chain reaction strategy. Annu. Rev. Biochem. 61, 131-156. 

Arnheim, M., and Erlich, H. (1992). Polymerase Chain Reaction Strategy. Anna Rev Biochem 61 131. 

Amheim N and Erlich H (1992) Polymerase Chain Reaction Strategy. Annual Reviews in Biochemistry 61 131-156. 

Both target and signal amplification systems have been successfully employed to detect and quantitate specific nucleic acid sequences in clinical specimens. Polymerase chain reaction (PCR), nucleic acid sequence-based amplification (NASBA), transcription-mediated amplification (TMA), strand displacement amplification (SDA), and ligase chain reaction (LCR) are all examples of enzyme-mediated, target amplification strategies that are capable of producing billions of... 

Schaefer, B.C. Revolutions in rapid amplification of cDNA ends New strategies for polymerase chain reaction cloning of full-length cDNA ends. Anal Biochem 227 255-273, 1995. 

The use of molecular probes to track specific microbes in the environment, specifically those not easily cultured, has been recently reviewed (95, 97, 98), The sensitivity of these probes may be further enhanced by using amplification strategies (e.g., polymerase chain reaction or PCR), to amplify segments of DNA from samples obtained from production systems (95, 99), However, gene probes for geosmin or MIB synthesis are not currently available. 

Adler M. Hapten labeling of nucleic acids for immuno-polymerase chain reaction applications, bioconjugation protocols Strategies and methods. In Niemeyer CM, editor. Methods in Molecular Biology. Humana Press, 2004 163-180. 

Fig. 5. (Opposite page) Polymerase chain reaction (PCR)-based expression of cDNA information using conventional method (A) and a new strategy using split-type primers (B). a. Design of the split-type primers for the introduction of the required UTRs into cDNA sequences, b and c. Expected PCR-generated DNAs and mRNA, respectively, d. PCR-generated DNA. e. A 10-pL aliquot from aPCR sample was used forthe 100-pL transcription reaction, and transcripts were analyzed, f. All of the transcript was used for batch-mode translation (50 pL, 4 h), and products were analyzed by autoradiography. (From ref. 36a.)...

One of the major facilitators of DNA research has been researchers ability to generate almost unlimited quantities (copies) of a target nucleic acid sequence by using polymerase chain reaction (PCR) techniques. Currently, however, there is not an equivalent technique for proteins, and so research techniques have to study the very small amount of molecules that are produced in vivo. Three strategies of interest in protein research are ... 

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